The role of leucine in the formation of beta amyloid peptide in cell culture SH-SY5Y of human neuroblastoma

• Grant number: 12/02060-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2012
• End date: July 31, 2014
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Antonio Herbert Lancha Junior
• Grantee: Fábio Medici Lorenzeti
• Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The brain tissue as well as all other tissues is subject to aging. This process goes over the age of a mature tissue, a tissue progressively degraded and disorganized, showing a progressive loss of neurons and oligodendrocytes during the course of life. During aging there is increased oxidative stress in neurons as manifested by protein oxidation and lipid peroxidation, among other factors that induce neurodegeneration. Neuronal oxidative stress is positively correlated with increased synthesis of the different isoforms of beta amyloid peptide which is responsible for the development of diseases such as dementia and Alzheimer's disease. In an animal model of diet enrichment with branched chain amino acids chronically seemed to be responsible for neuronal oxidative stress. Thus, this study will try to evaluate the role of leucine in the formation of beta amyloid peptide in vitro model for human cell culture. For this, we studied SH-SY5Y cells from human neuroplasm maintained in different culture media in order to evaluate the role of leucine in the formation of beta amyloid peptide. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LORENZETI, Fábio Medici. The role of leucine in the modulation of beta amyloid peptide activity in SH-SY5Y cells. 2014. Master's Dissertation - Universidade de São Paulo (USP). Escola de Educação Física e Esportes (EEFE/BT) São Paulo.