Analysis of osteogenesis, cytotoxicity and genotoxicity of bone substitutes in osteoblasts derived from human alveolar bone

• Grant number: 12/02649-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2012
• End date: December 31, 2013
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Samira Esteves Afonso Camargo
• Grantee: Noala Vicensoto Moreira Milhan
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The substitute bone integration is due to the integration cell-material. The objective of this study is to investigate in vitro the behavior of human osteoblastic cells and osteogenesis on two types of bone grafts: a xenogenic - Bio-oss ® (Geistlich) and an alloplastic - resorbable tissue replacement (R.T.R., Septodont). The followed aspects will be evaluated: cell adhesion, cell viability, total protein content, alkaline phosphatase activity, mineralized matrix nodular formation and genotoxicity analysis. The data will be evaluated by ANOVA and Tukey's tests, all with a significance level of 5%.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MILHAN, Noala Vicensoto Moreira. Análise dos indicadores da osteogênese, citotoxicidade e genotoxicidade de substitutos ósseos em osteoblastos-like. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.