Liquid crystalline nanodispersions as a carrier of siRNA in the topical treatment of psoriasis: In vitro and in vivo evaluation and elucidation of the mechanisms of cellular uptake

• Grant number: 12/09125-8
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): September 01, 2012
• Effective date (End): May 31, 2016
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal researcher: Maria Vitória Lopes Badra Bentley
• Grantee: Lívia Vieira Depieri
• Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Gene therapy by RNA interference (RNAi) is a post-transcriptional silencing process that can suppress the expression of a particular gene. The RNA interference is a promising gene therapy approach for the treatment of many severe diseases that have no cure or well-defined treatments. However, the development of clinically appropriate, safe and effective delivery systems is necessary to enable this new therapy, since obstacles in the in vivo administration and distribution committed the clinical use of siRNAs (small interfering RNA). In addition, the topical delivery of siRNAs appears as a promising alternative for the treatment of cutaneous pathologies. In this context, this project aims the topical administration of liquid-crystalline nanodispersions, composed of monoolein, oleic acid and water, incorporated with adjuvants cationic polyethyleneimine and oleylamine, for carrier siRNA in the treatment of psoriasis with evaluation in vitro/in vivo of their activity and effectiveness, as well as investigation the mechanisms of cellular uptake of this delivery system.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEPIERI, LIVIA VIEIRA; BORGHETI-CARDOSO, LIVIA NEVES; CAMPOS, PATRICIA MAZUREKI; OTAGUIRI, KATIA KAORI; MOURA DE CARVALHO VICENTINI, FABIANA TESTA; LOPES, LUCIANA BIAGINI; VIEIRA FONSECA, MARIA JOSE; VITORIA LOPES BADRA BENTLEY, M. RNAi mediated IL-6 in vitro knockdown in psoriasis skin model with topical siRNA delivery system based on liquid crystalline phase. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 105, p. 50-58, AUG 2016. Web of Science Citations: 15.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DEPIERI, Lívia Vieira. Liquid crystalline nanodispersions as a siRNA carrier in the topical treatment of psoriasis: Evaluation in in vitro and in vivo models. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.