Production of human recombinant erythropoietin in mice milk

• Grant number: 05/01392-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2006
• End date: February 28, 2009
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: José Antonio Visintin
• Grantee: Camilla Mota Mendes
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The aim of this study will be establish techniques and conditions to produce transgenic mice, having as first model the human erythropoietin gene. The vector with specific promoter for milk expression will be amplified and will have the human erythropoietin gene inserted. The vector with the gene will be introduced in embryonic stem cells culture. The embryonic stem cells will be aggregated to embryos flushed from superovulated mice. After 24 hours of in vitro culture well-developed embryos will be transferred to pseudopregnant recipients. The offspring (chimeras) will be breeded and its newborns will have their DNA analyzed to verify the presence of human erythropoietin gene. The positive animals (founders) will be breeded and the borned females will be studied for human erythropoietin production in milk. Human erythropoietin will be quantified and its activity will be also tested. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MENDES, Camilla Mota. Production of recombinant human erythropoietin in transgenic mice Milk. 2009. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.