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Evaluation of the effect of adding fluorescent agent (Rhodamine B) in non-simplified adhesive systems

Grant number: 11/14971-2
Support type:Scholarships in Brazil - Master
Effective date (Start): October 01, 2012
Effective date (End): April 30, 2013
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Linda Wang
Grantee:Odair Bim Júnior
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Rhodamine B is a fluorescent dye commonly used in dentistry for assessing several aspects of adhesive restorations over confocal laser scanning microscopy. By adding rhodamine in adhesive systems, it is possible to apprise characteristics of resin components distribution throughout previously demineralized dentin (the hybrid layer micromorphology, extent and amount of resin tags and adhesive layer thickness) as well as detecting potential defects or changes in adhesive interface (Griffiths, 1999; Walter et al, 2008; Ding et al, 2010; Sampaio et al, 2011). However, dental field literature reveals lack of methodological agreement on dye dissolution in bonding systems (D'Alpino et al, 2006a). The current study intends to estimate the serviceable rhodamine B concentration in simplified bonding systems regarded as gold standard in their categories: Adper" Scotchbond" Multi-Purpose (MP) - etch-and-rinse three steps- and Clearfil" SE Bond (CSE) - a self-etching two steps, considering the null hypotheses that there is no significant difference in tensile bond strength after addition of rhodamine B in concentrations to be determined during the experiment itself, according to each adhesive system evaluated, and that there is no difference in tensile bond strength over time, due to these treatments for such bonding systems. Stage A - Determination of the proportion between rhodamine B mass and adhesive/primer volumes, according to the adhesive system over assessment, since it is enough for full viewing the dye in prepared specimens under microscopic evaluation. Stage B - Using the concentration values determined in previous stage, other properties are intended to be evaluated, especially, the tensile bond strength of adhesive to dentin in specimens prepared for micro-tensile bond test. Stage C - Evaluation of sorption and solubility of MP and CSE's bond components, subjecting them to cycling and desiccation. Micro-tensile bond test results will be correlated with analysis of variance (ANOVA) and paired t-test for each bonding system. In addition, the specimens fractured during micro-tensile bond test will be qualitatively analyzed in scanning electron microscope for determining the fracture types. Results obtained with sorption and solubility procedures will be analyzed through one-way analysis of variance, if there is normal distribution of data, and Tukey's test. Significance level of 5% is considered.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BIM JUNIOR, ODAIR; CEBIM, MARCO A.; ATTA, MARIA T.; MACHADO, CAMILA M.; FRANCISCONI-DOS-RIOS, LUCIANA F.; WANG, LINDA. Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface. Microscopy and Microanalysis, v. 23, n. 1, p. 122-130, FEB 2017. Web of Science Citations: 2.
WANG, LINDA; BIM JUNIOR, ODAIR; DE OLIVEIRA LOPES, ADOLFO COELHO; FRANCISCONI-DOS-RIOS, LUCIANA FAVARO; MAENOSONO, RAFAEL MASSUNARI; PERLATTI D'ALPINO, PAULO HENRIQUE; HONORIO, HEITOR MARQUES; ATTA, MARIA TERESA. Water interaction and bond strength to dentin of dye -labelled adhesive as a function of the addition of rhodamine B. Journal of Applied Oral Science, v. 24, n. 4, p. 317-324, JUL-AUG 2016. Web of Science Citations: 4.
LINDA WANG; ODAIR BIM JÚNIOR; ADOLFO COELHO DE OLIVEIRA LOPES; LUCIANA FÁVARO FRANCISCONI-DOS-RIOS; RAFAEL MASSUNARI MAENOSONO; PAULO HENRIQUE PERLATTI D’ALPINO; HEITOR MARQUES HONÓRIO; MARIA TERESA ATTA. Water interaction and bond strength to dentin of dye-labelled adhesive as a function of the addition of rhodamine B. Journal of Applied Oral Science, v. 24, n. 4, p. -, Ago. 2016.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
JÚNIOR, Odair Bim. Estratégias para adição de rodamina B em sistemas adesivos. 2013. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru Bauru.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.