Nephrocalcinosis is induced by hyperoxaluria: experimental model in rats

• Grant number: 11/22352-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2012
• End date: August 31, 2013
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: João Luiz Amaro
• Grantee: Eloá Siqueira Magalhães
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Nephrocalcinosis inducers may be used in different concentrations and periods. They may also cause variables grades of renal parenchyma calcifications. Thus, one should use better agents and concentrations that are not elucidated. The objective of this study is to evaluate the intensity of nephrocalcinosis, which is induced by two different products; however, one must also take into account the level of nephrotoxicity. Materials and methods: 40 Sprague-Dawly rats will be randomly distributed into four groups: a) Group I (Clinical control, n=10): animals are not subjected to any intervention and they will be used as the clinical control; b) Group II (Ethylene Glycol at 0.5% + Vitamin D3, n=10): Ethylene Glycol at 0.5% will be diluted in water and then offered "ad libitum" to the animals. The Vitamin D3 (Cholecalciferol) at a dose of 0.5 ¼M will be dissolved in 1 ml of oil and inoculated by gavage once a day; c) Group III (Ethylene Glycol at 1.25%, n=10): Ethylene Glycol at 1.25% will be diluted in water and administered "ad libitum" to the animals; d) Group IV (Hydroxy-L-Proline at 5%, n=10): Hydroxy-L-Proline at 5% will be diluted in water and given "ad libitum" to the animals. Five animals from each group will be sacrificed after a follow-up of one week (Moment M1) and five others will be sacrificed after completing 4 weeks (Moment M2). All of the animals will take part in metabolic, histological, and oxidative stress studies. Statistical analysis of the sample will be applied to the Levene Test, and to various homogeneity and Kolmogorov-Smirnov tests in order to determine their normalities. Then, depending on the conditions of homogeneity and normality of the sample, they will be applied to non-parametric and parametric tests. All comparisons were performed with 5% level of significance.(AU)