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Evaluation of the role of pyridoxamine in the prevention of nephrolithiasis in rats induced by hyperoxaluria

Grant number: 13/12535-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2013
Effective date (End): August 31, 2014
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Paulo Roberto Kawano
Grantee:Matheus Augusto Callegari
Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Aims: calcium oxalate is the main component involved in metabolic nephrolithiasis. Different authors have used hyperoxaluria to study the deposition of calcium oxalate crystals in the renal tubules in rats, reproducing models in which have contributed significantly to understanding the mechanisms involved in nephrolithiasis. Pyridoxamine, a component of vitamin B6, has been suggested as a potential therapeutic agent for decreasing hyperoxaluria. This study aims to evaluate the intensity of nephrocalcinosis caused by the inducing agent, and the role of pyridoxamine in hyperoxaluria induced in an experimental rat model. Materials and Methods: It will be used 60 male rats Sprague-Dawley and housed in metabolic cages, selected randomly distributed into four groups: group I (Clinical control, n = 15) the animals are not subjected to any intervention, and will be used as control, group II (Ethylene Glycol + 0.5% Vitamin D3, n = 15) in which Ethylene Glycol 0.5% is diluted in water and offered "ad libitum" to the animals. Vitamin D3 (Cholecalciferol) at a dose of 0.5 microM be dissolved in 1 ml of oil and administered by gavage one time per day; GROUP III (Ethylene Glycol 1.25% Vitamin D3 + + pyridoxamine, n = 15) animals receive the same drug offered to group II plus pyridoxamine gavage to be dissolved in 1 ml of water daily, according to the same weight (180 mg/kg body weight/day) gROUP IV (pyridoxamine, n = 15) via gavage at a dose of 180mg/kg/dia. The sacrifice occurs at the end of 28 days. All animals will be performed the metabolic study, histological analysis, determination of oxidative stress, and spectroscopic dosage of calcium in the renal parenchyma for comparison between groups and discussion with the literature. (AU)

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