Antimicrobial efficacy of a experimental dentifrice based of Ricinus communis on contamined specimens of soft liner

• Grant number: 11/23630-4
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2012
• Effective date (End): August 31, 2013
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Cláudia Helena Lovato da Silva
• Grantee: Luciano Trevizan Martins
• Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

In order to promote the oral health of usurers of removable dentures, it is necessary to develop effective and easy methods for control biofilm. Thus, aim of this study is to evaluate the antimicrobial action of a experimental dentifrice based Ricinus communis against biofilm formed on the surface of resilient denture liner specimens. The antimicrobial activity will be assessed in relation other three dentifrices against specific microorganisms (Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Bacillus subtilis, Enterococcus faecalis, Candida albicans and Candida glabrata). The study will have a positive and a negative control so that will be formed six groups totaling 210 specimens. Specimens of Mucopren soft resilient reline will be made from an acrylic matrix hollow (15mm diameter x 3mm thick), sterilized with ethylene oxide, contaminated by specified microorganims for biofilm formation, brushed, washed, incubated in appropriate culture medium, which will be sown in Petri dishes in order to verify whether or not the growth of microorganisms and counting the CFU.