Dental caries is a multifactorial disease still considered a general problem of public health, so prevention methods are constant targets of scientific studies. The grape seed extract (ESU) contains proanthocyanidins and has several clinical applications by acting as antioxidants and act positively in the degradation of demineralized dentin collagen. Therefore, the purpose of this study is to assess the ability of the ESU in preventing demineralization of enamel and dentin under caries challenge by analyzing the surface microhardness and depth, as well as qualitative assessment of the depth of the lesion by means of polarized light microscopy (MLP). Forty five bovine enamel fragments (6-mm²) and 45 dentin root fragments (6-mm²) will be polished to obtain a plane surface. Then the fragments are randomly divided into three groups (n = 15), according to the treatments: 1) GC-control (untreated), 2) GSU-grape seed extract and 3) GF-fluoride - 1000ppm. The groups will be subjected to a pH cycling (demineralization and remineralization): 10 minutes in the treatment solution (GC GF; GSU), 30 minutes in the demineralization solution and 10 minutes in buffered solution with 6 repetitions daily for 8 days. After cycling, the surface microhardness of enamel and dentin will be analyzed by carrying out three rows of 5 indentations each, followed by the preparation of specimens for analysis of the depth of the lesion by MPL. The obtained values of hardness and size of lesions will be subjected to statistical analysis with significance level of 5%.
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