Synergistic action of propolis constituents on cytokines production and bactericidal activity of monocytes

• Grant number: 12/14336-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2012
• End date: September 30, 2013
• Field of knowledge: Biological Sciences - Pharmacology - Ethnopharmacology
• Principal Investigator: José Maurício Sforcin
• Grantee: Eliza de Oliveira Cardoso
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Propolis is a resinous product made by bees from different plants. In recent years, its biological activities, such as antibacterial, antiviral, antifungal, anti-inflammatory, and immunomodulatory have been intensely investigated. Data from the literature concerning the action of propolis and its isolated components were obtained mainly in vitro or using experimental animals. Our project aims to understand the synergistic action of propolis components using human monocytes from healthy individuals. The action of three phenolic compounds found in propolis (cinnamic acid, p-coumaric acid, and caffeic acid), and combinations of these constituents will be tested according to the concentration found in propolis, to identify the synergistic action responsible for propolis action. A possible cytotoxic activity of the compounds will be assessed on these cells. The production of anti- and pro-inflammatory cytokines (IL-10 and TNF-±) by monocytes will be evaluated after incubation with the compounds, as well as the bactericidal activity of these cells against Escherichia coli. The comprehension of the mechanism of action of propolis and its compounds may be useful for the development of new products by the pharmaceutical industry since propolis has been widely used by humans.(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOSO, ELIZA DE OLIVEIRA; CONTI, BRUNO JOSE; SANTIAGO, KARINA BASSO; CONTE, FERNANDA LOPES; GARCIA OLIVEIRA, LUCAS PIRES; HERNANDES, RODRIGO TAVANELLI; GOLIM, MARJORIE DE ASSIS; SFORCIN, JOSE MAURICIO. Phenolic compounds alone or in combination may be involved in propolis effects on human monocytes. Journal of Pharmacy and Pharmacology, v. 69, n. 1, p. 99-108, JAN 2017. (12/14336-8, 14/13227-6)