Gene expression characterization of signal transduction pathways in the remote myocardium after infarction induced by left ventricular ablation and coronary artery occlusion in rats

• Grant number: 12/15808-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2012
• Effective date (End): September 30, 2015
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal researcher: José Antonio Silva Junior
• Grantee: Eduardo Tadeu Santana
• Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated research grant: 09/54225-8 - The pathophysiology of congestive heart failure: from the organ to tile molecules, AP.TEM

Abstract

The ligation of anterior descending coronary artery is the most widely used experimental model to induce myocardial infarction (MI) in rodents. The high mortality in the acute phase and the heterogeneity of the size of MI obtained are recognized drawbacks in this model. In an attempt to solve the problem, recently was developed a new experimental model of MI in rats based on the application of current myocardial ablation radio frequency. The new technique allowed one to obtain homogeneous and MI with sizes significantly reduce acute mortality. It should be noted also that the structural and functional cardiac changes evoked by ablation were similar to those observed in animals with MI induced by coronary ligation. In this project, it is proposed to evaluate the gene expression of 15 different signal transduction pathways (signals cell survival, cell cycle, stress, apoptosis, molecules related to calcium signaling, cell growth factors, transcription factors, angiogenesis, inflammation and hypoxia) in the left ventricle of rats with MI promoted by experimental models of coronary occlusion and ablation. The proposal is based on the fact that, with the identification of reprogramming of gene expression will be possible to consolidate that, besides the similarity in structural and functional remodeling of the myocardium, there is between the two models, similar modifications of the transcriptional response of genes that govern the ventricular remodeling after MI. Furthermore, the results of this design will make specific information about changes in signal transduction pathways of each experimental model which leads to unfavorable myocardial remodeling.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, EDUARDO T.; FELICIANO, REGIANE DOS SANTOS; SERRA, ANDREY J.; BRIGIDIO, EDUARDO; ANTONIO, EDNEI L.; TUCCI, PAULO J. F.; NATHANSON, LUBOV; MORRIS, MARIANA; SILVA, JR., JOSE A. Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents. FRONTIERS IN PHYSIOLOGY, v. 7, NOV 25 2016. Web of Science Citations: 2.