Arrabidaea chica Verlot ethanolic extract activity on fibroblasts, keratinocytes, osteoblasts and osteoclasts under a bisphosphonate treatment

• Grant number: 11/21006-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 01, 2012
• End date: April 30, 2013
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Mary Ann Foglio
• Grantee: Patrícia Maria Wiziack Zago
• Supervisor: Deepak Saxena
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: New York University, United States
• Associated to the scholarship: 10/07781-0 - Arrabidaea chica Verlot: in vitro evaluation of toxicity, antimicrobial and cytoprotective activities from standardized free crude extract combined with zoledronic acid, and microencapsulation studies, BP.DR

Abstract

Bisphosphonates are medications used to treat bone disorders such osteoporosis and used as treatment adjuncts for metastatic malignancies and multiple myeloma. These drugs inhibit osteoclast function and promote fibroblasts and keratinocytes apoptosis, causing mucosal ulcerations. It has been observed the development of osteonecrosis of the jaws in patients under a chronic bisphosphonate use, which is clinically characterized by persistent bone exposure in the maxillofacial region. Arrabidea chica (Humb. & Bonpl.) Verlot, is a species whose healing, fibroblast proliferation, antiulcerogenic and antioxidant activities of its crude extract has been proven in studies at CPQBA-Unicamp (Jorge et al., 2008). The necessity of a osteonecrosis of the jaws treatment, without losing the bisphosphonates benefits, motivated this research to evaluate the activity of free and microencapsulated A.chica ethanolic extract, on the proliferation and activity of fibroblasts, keratinocytes, osteoblasts and osteoclasts treated with that medication. Cells will be treated with zoledronic acid at four different concentrations, than free or microencapsulated A.chica ethanolic extract will be added. After this period, they will be stained by MTT assay. Cellular apoptosis, genes quantification, growth factors and collagen production will be performed by Real-time PCR, Western blot and ELISA (evaluation of type I collagen production by fibroblasts and osteoblasts) assays. A cell migration assay will be performed with epithelial cells. Results will be statistically analyzed by analysis of variance (ANOVA) with significance level of p d 0.05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WIZIACK ZAGO, PATRICIA MARIA; OLIVEIRA SOUSA, ILZA MARIA; SERVA-MEDINA, LEILA; JORGE, MICHELLE PEDROZA; LIMA NETO, LIDIO GONCALVES; HASS, VIVIANE; LI, XIN; GOIS RUIZ, ANA LUCIA TASCA; SAXENA, DEEPAK; FOGLIO, MARY ANN. Standardized Arrabidaea chica Extract Shows Cytoprotective Effects in Zoledronic Acid-Treated Fibroblasts and Osteoblasts. CLINICAL COSMETIC AND INVESTIGATIONAL DENTISTRY, v. 12, p. 327-333, 2020. (11/09127-8, 10/07781-0, 11/21006-1)