Global gene expression pattern of Bos taurus indicus and Bos taurus taurus in vitro and in vivo produced embryos

• Grant number: 12/02333-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 30, 2012
• End date: November 29, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Fernanda da Cruz Landim
• Grantee: Mateus José Sudano
• Supervisor: Peter J. Hansen
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of Florida, Gainesville (UF), United States

Abstract

The proposal objectives are: Experiment I - Study the effect of subspecies (Bos taurus indicus vs. Bos taurus taurus), different embryo culture media (SOFaa + 2.5% FCS versus SOFaa + 2.5% FCS + 0.3 µM PES) and embryo condition (fresh versus vitrified) on global gene expression pattern (microarray) cryotolerance, lipid content (Sudan Black), and lipid fingerprint mass spectrometry profile (MALDI-TOF MS) of in vitro produced embryos (OPU-PIV). Experiment II - Evaluate the effect of different embryo culture media (SOFaa without FCS; SOFaa + 2.5% FCS; SOFaa + 2.5% FCS + 0.3 µM PES, and SOFaa + 10% FCS) and embryo condition (fresh versus vitrified) on embryo development, lipid content, lipid fingerprint mass spectrometry profile, cryotolerance, and relative gene expression pattern of in vitro produced embryos (PIV). Experiment III - Analyse the effect of subspecies (Bos taurus indicus vs. Bos taurus taurus) and embryo condition (fresh and vitrified) on global gene expression pattern, cryotolerance, lipid content and lipid fingerprint mass spectrometry profile of in vivo produced embryos (SOV). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUDANO, MATEUS J.; SANTOS, VANESSA G.; TATA, ALESSANDRA; FERREIRA, CHRISTINA R.; PASCHOAL, DANIELA M.; MACHADO, RUI; BURATINI, JOSE; EBERLIN, MARCOS N.; LANDIM-ALVARENGA, FERNANDA D. C.. Phosphatidylcholine and Sphingomyelin Profiles Vary in Bos taurus indicus and Bos taurus taurus In Vitro- and In Vivo-Produced Blastocysts. BIOLOGY OF REPRODUCTION, v. 87, n. 6, DEC 2012. (11/06191-7, 10/09922-0, 12/02333-4)

TATA, ALESSANDRA; SUDANO, MATEUS J.; SANTOS, VANESSA G.; LANDIM-ALVARENGA, FERNANDA D. C.; FERREIRA, CHRISTINA R.; EBERLIN, MARCOS N.. Optimal single-embryo mass spectrometry fingerprinting. Journal of Mass Spectrometry, v. 48, n. 7, p. 844-849, JUL 2013. (11/06191-7, 12/02333-4)

SUDANO, MATEUS J.; CAIXETA, ESTER S.; PASCHOAL, DANIELA M.; MARTINS, JR., ALICIO; MACHADO, RUI; BURATINI, JOSE; LANDIM-ALVARENGA, FERNANDA D. C.. Cryotolerance and global gene-expression patterns of Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 26, n. 8, p. 1129-1141, 2014. (10/09922-0, 12/02333-4)