Delopment and evaluation of an assay based in the loop mediated isothermal amplification of DNA (lamp) technique for the detection of Ehrlichia canis in naturally infected dogs

• Grant number: 12/15940-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2012
• End date: May 31, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Mozart de Azevedo Marins
• Grantee: Vitor Caressato Pinhanelli
• Host Institution: Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil

Abstract

The isothermal amplification of DNA loop-mediated (LAMP) is a technique that can be applied to the development of diagnostic tests for the detection of parasites in biological samples. Although PCR may be applied successfully for this purpose, time and cost for running the assays are still impeditive for large-scale use in routine laboratories. In this project a LAMP assay will be developed for the detection of the bacterium Ehrlichia canis, the agent of canine monocytic ehrlichiosis (CME), which is one of the major diseases transmitted by ticks to dogs. The test will be based on amplification of the p30 gene and compared to PCR and nested PCR assays based on this gene and 16S ribosomal RNA gene, as described in the literature. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINHANELLI, V. C.; COSTA, P. N. M.; SILVA, G.; AGUIAR, D. M.; SILVA, C. M. L.; FACHIN, A. L.; MARINS, M.. Development and evaluation of a loop-mediated isothermal amplification assay for detection of Ehrlichia canis DNA in naturally infected dogs using the p30 gene. Genetics and Molecular Research, v. 14, n. 4, p. 17885-17892, 2015. (12/15940-6, 12/01235-9)