Metabolomic and proteomic profile and cell proliferation and apoptosis in Walker tumour-bearing rats under pregnancy hormones and leucine-rich diet

• Grant number: 12/06955-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2012
• End date: October 27, 2015
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Maria Cristina Cintra Gomes Marcondes
• Grantee: Laís Rosa Viana
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Pregnancy is characterized by many maternal physiological adaptations related to hormonal variations levels, especially oestrogen, progesterone, hCG and prolactin, which are essential to support foetal growth. Pregnancy hormones influence and modulate the breast cancer incidence and growth. However, there are few source and controversial data about the influence of these hormones on tumour growth. For this reason, this study aim to investigate the influence of pregnancy hormones - oestrogen, progesterone, hCG and prolactin - and also the influence of nutritional supplementation with leucine on Walker 256 tumour growth in vitro and in vivo. In vivo studies will be conducted in Wistar rats, which will be distributed in eight different groups: control (C), Walker tumour-bearing (W), pregnant (P) and tumour-bearing pregnant rats (PW); additional four groups subjected to leucine-rich diet; leucine (L), leucine tumour-bearing (LW), leucine pregnant rat (LP), leucine tumour-bearing pregnant rat (LPW). The inoculation of Walker 256 tumour occurs in the second day of pregnancy, and after 19 days of experiment, the animals will be sacrificed to analyse the metabolomic profile, serum hormones and cytokines content and tumour tissue will be analysed the profiles metabolomic, proteomic expression of proteins related to signalling processes, cell proliferation and apoptosis. In vitro experiments, Walker 256 cells culture will be treated with pregnant hormones and will be accessed and evaluated these cells proliferation and cytotoxicity under pregnant hormones, and also the effects on cell signalling proteins and apoptosis. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIANA, LAIS ROSA; TOBAR, NATALIA; BRANDT BUSANELLO, ESTELA NATACHA; MARQUES, ANA CAROLINA; DE OLIVEIRA, ANDRE GUSTAVO; LIMA, TANES I.; MACHADO, GABRIELLY; CASTELUCCI, BIANCA GAZIERI; RAMOS, CELSO DARIO; BRUNETTO, SERGIO Q.; et al. Leucine-rich diet induces a shift in tumour metabolism from glycolytic towards oxidative phosphorylation, reducing glucose consumption and metastasis in Walker-256 tumour-bearing rats. SCIENTIFIC REPORTS, v. 9, OCT 29 2019. (15/21890-0, 12/06955-0, 17/02739-4, 14/13334-7)

VIANA, LAIS ROSA; LOPES-AGUIAR, LEISA; ROSOLEN, RAFAELA ROSSI; DOS SANTOS, ROGERIO WILLIANS; CINTRA GOMES-MARCONDES, MARIA CRISTINA. H-1-NMR Based Serum Metabolomics Identifies Different Profile between Sarcopenia and Cancer Cachexia in Ageing Walker 256 Tumour-Bearing Rats. METABOLITES, v. 10, n. 4, APR 2020. (15/21890-0, 12/06955-0, 17/02739-4, 14/13334-7, 19/20558-2)

VIANA, LAIS ROSA; TOBAR, NATALIA; BUSANELLO, ESTELA; MARQUES, ANA CAROLINA; LIMA, TANES; MACHADO, GABRIELLY; CASTELUCCI, BIANCA; RAMOS, CELSO DARIO; SERGIO; SILVEIRA, LEONARDO REIS; et al. Leucine-rich diet changes tumor metabolism, reducing glucose consumption and metastasis in Walker 256 tumor-bearing rats. FASEB JOURNAL, v. 33, p. 2-pg., 2019-04-01. (12/06955-0, 15/21890-0, 17/02739-4, 14/13334-7)

VIANA, LAIS ROSA; CANEVAROLO, RAFAEL; PERINA LUIZ, ANNA CAROLINE; SOARES, RAQUEL FRIAS; LUBACZEUSKI, CAMILA; DE MATTOS ZERI, ANA CAROLINA; CINTRA GOMES-MARCONDES, MARIA CRISTINA. Leucine-rich diet alters the H-1-NMR based metabolomic profile without changing the Walker-256 tumour mass in rats. BMC CANCER, v. 16, OCT 3 2016. (12/06955-0, 13/16115-1, 10/00209-9)

VIANA, LAIS ROSA; GOMES-MARCONDES, MARIA CRISTINA C.. Leucine-Rich Diet Improves the Serum Amino Acid Profile and Body Composition of Fetuses from Tumor-Bearing Pregnant Mice. BIOLOGY OF REPRODUCTION, v. 88, n. 5, MAY 2013. (12/06955-0, 10/00209-9, 09/11982-3)

VIANA, LAIS ROSA; CINTRA GOMES-MARCONDES, MARIA CRISTINA. A Leucine-Rich Diet Modulates the Tumor-Induced Down-Regulation of the MAPK/ERK and PI3K/Akt/mTOR Signaling Pathways and Maintains the Expression of the Ubiquitin-Proteasome Pathway in the Placental Tissue of NMRI Mice. BIOLOGY OF REPRODUCTION, v. 92, n. 2, FEB 1 2015. (12/06955-0, 10/00209-9, 09/11982-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VIANA, Laís Rosa. Effects of nutritional supplementation with leucine in serum and tumour metabolic profile of Walker 256-tumour bearing rats. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.