Evaluation of adhesive-dentin interface, 2D and 3D imaging of marginal adaptation of class I composite restorations

• Grant number: 12/18583-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: March 01, 2013
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Marcelo Giannini
• Grantee: Patricia Makishi
• Supervisor: Junji Tagami
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: Tokyo Medical and Dental University (TMDU), Japan
• Associated to the scholarship: 11/22093-5 - Evaluation of adhesive/dentin interface, 2D and 3D imaging of marginal adaptation of Class I composite restorations and their bond-strength, BP.PD

Abstract

Objectives: To evaluate different restorative materials to superficial-dentin and their marginal adaptation in class I cavity. Methods: Round-shaped class I cavities (3 mm diameter x 1.5 mm depth) will be prepared in the oclusal surface of fresh extracted human third-molars, with margins located in superficial dentin. Four adhesive systems will be used and evaluated in this study; one two-step total-etch adhesive system (Adper Single Bond 2), one two-step self-etch adhesive system (Clearfil SE Bond) and two one-step adhesives (G-Bond, Clearfil S3 Plus), restored a with compiste resin (Clearfil Majesty Posterior) or a low-shrinkage composite with its proper adhesive (Filtek P-90, Silorane Adhesive System). The specimens will be immersed in water (24 h, 37°C) or thermal stress cycling (10000 cycles, 5°C and 55°C). Ammoniacal silver nitrate solution will be used to evaluate the marginal sealing ability and 3D scans (4 mm x 4 mm x 2.6 mm) of each restoration will be obtained using Optical Coherence Tomography (OCT). 2D images will be obtained from the 3D scans (n=15/group) and their marginal adaptation will be analyzed. Area fraction of pixels with high brightness values at the interfacial zone will be calculated using a digital image analysis software. Results: The results of marginal adaptation will be submitted to two-way ANOVA and Tukey test for statistical analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRONZA, BRUNA MARIN; MAKISHI, PATRICIA; SADR, ALIREZA; SHIMADA, YASUSHI; SUMI, YASUNORI; TAGAMI, JUNJI; GIANNINI, MARCELO. Evaluation of bulk-fill systems: microtensile bond strength and non-destructive imaging of marginal adaptation. Brazilian Oral Research, v. 32, 2018. (12/18583-0, 13/05247-4)

MAKISHI, PATRICIA; THITTHAWEERAT, SUPPASON; SADR, ALIREZA; SHIMADA, YASUSHI; MARTINS, ADRIANO LUIS; TAGAMI, JUNJI; GIANNINI, MARCELO. Assessment of current adhesives in class I cavity: Nondestructive imaging using optical coherence tomography and microtensile bond strength. Dental Materials, v. 31, n. 9, p. E190-E200, SEP 2015. (12/18583-0, 11/22093-5)

FRONZA, BRUNA MARIN; MAKISHI, PATRICIA; SADR, ALIREZA; SHIMADA, YASUSHI; SUMI, YASUNORI; TAGAMI, JUNJI; GIANNINI, MARCELO. Evaluation of bulk-fill systems: microtensile bond strength and non-destructive imaging of marginal adaptation. BRAZILIAN ORAL RESEARCH, v. 32, p. 12-pg., 2018-01-01. (13/05247-4, 12/18583-0)