Evaluation of interleukin-1 beta transcript profile in Danio rerio (zebrafish) brain after seizure evoked by Pentylenetetrazole at different age

• Grant number: 12/20113-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2012
• End date: November 30, 2013
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Cláudia Vianna Maurer Morelli
• Grantee: Danielle Miyuki Nakata
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The main aim of this study is to investigate whether there is a direct relationship between age, in which the seizure is induced, and the transcript levels of il1b gene. To achieve this objective, seizures will be evoked by Pentylenetetrazole in zebrafish at 15 and 30 days post-fertilization, and the brain transcript levels quantified by Real-Time PCR. This study is part of a larger project that objectives to analyze the temporal transcript pattern of il1b gene at seven days post-fertilization, as well as investigate the neuronal loss, in zebrafish brains after a seizure. Certainly, the knowledge obtained from this study will contribute to better characterizing the zebrafish as a model for epilepsy studies.(AU)