Sequencing and comparative analysis of genes involved in feeding preference in the Calliphoridae family (Diptera: Calliphoridae)

• Grant number: 12/19987-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2012
• End date: November 30, 2013
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Tatiana Teixeira Torres
• Grantee: Raquel Dietsche Monfardini
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The order Diptera is one of the most diversified orders of insects and it is important for human and animal health, as it includes several species of parasites and vectors. The Calliphoridae family is a good example for the investigatin of the evolution of parasitism, since this life-style has evolved independently at least three times in its evolutionary history.The condition caused by the Calliphoridae species is known as myiasis, which is the infestation of vertebrates by dipterous larvae which feed on dead or living tissues of the host. Within this family, the genera Chrysomya and Cochliomyia include both parasitic species (primary myiasis) and necrophagous (secondary myiasis).The study of genes involved in feeding habits, previously characterized in model species, will generate insights on the evolution of parasitism within the Calliphoridae family. This study is also of potential applied interest as can reveal new targets in order to control the infestation of ectoparasites, which is highly disarable as there have been reported cases of resistance to the current methods.Therefore, the objectives of this project include the sequencing of the coding sequence of five genes (for, mvl, cyp6g2, gdh e s6k) in parasitic and necrophagous species of the genera Chrysomya and Cochliomyia, and analyze whether the different feeding habits are corelated to change in function of their products and/or in their expression.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

CARDOSO, GISELE ANTONIAZZI; TONUS MARINHO, MARCO ANTONIO; MONFARDINI, RAQUEL DIETSCHE; LIMA DE AZEREDO ESPIN, ANA MARIA; TORRES, TATIANA TEIXEIRA. Evolution of genes involved in feeding preference and metabolic processes in Calliphoridae (Diptera: Calyptratae). PeerJ, v. 4, OCT 27 2016. (12/19987-7, 14/13933-8, 12/23200-2, 09/13463-3, 14/01600-4)