Study of gamma delta T cells in maintenance of maternal tolerance in pregnancy in cattle

• Grant number: 12/11212-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2012
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Daniele dos Santos Martins
• Grantee: Ana Carolina Furlanetto Mançanares
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The establishment and maintenance of maternal tolerance to fetal antigens are essential for successful pregnancy in animals Eutheria. The gamma delta T cells (³´T) are present in the endometrium / decidua during pregnancy in humans and mice. The amount of this gamma delta T cells in the uterus appears to increase with advancing gestation. The objectives of this project are to identify the presence of ³´T cells in the uterine endometrium in pregnant and non-pregnant cows; evaluate the population dynamics of ³´T cells in peripheral blood in early (around day 33 after insemination) and in the peripartum period cytometric flow, and analyzing cytokine expression profile of purified samples of ³´T cells by flow cytometry by means of real time quantitative PCR comparing cells from peripheral blood of pregnant cows with samples of cells from non-pregnant cows.The hypothesis of this project that there is a ³´T cell recruitment to the endometrium throughout gestation and express cytokines that promote the establishment and maintenance of maternal tolerance to fetal antigens during pregnancy in cattle.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MANÇANARES, Ana Carolina Furlanetto. Study of gamma delta t cells in maintenance of maternal tolerance in pregnancy in cattle. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.