Characterization of tumor stem cells after inoculation in nude mice

• Grant number: 12/20622-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: February 01, 2013
• End date: January 31, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Fabio Daumas Nunes
• Grantee: Nathália Paiva de Andrade
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Oral squamous cell carcinoma (OSCC) is the most prevalent neoplasia of oral cavity both in the world and in Brazil and its prognosis has not changed despite advances in knowledge of this neoplasm. We know that is a genetic disease that develops through multiple mutations which cause deregulation of the basic functions of the cell. Recently, was reported that head and neck squamous cell carcinomas present a subpopulation of tumoral initiators cells that apparently correspond to malignant stem cells, also responsible for tumor growth. This knowledge raised the perspective of new therapeutic approaches, and a better understanding of tumor development mechanisms. The main purpose of the present study is the isolation and characterization of oral squamous cell carcinoma stem cells from established cell lines using specifics cell molecules. Additionally, will be analyzed the tumorigenesis potential of a cell lineage and oral squamous cell carcinoma stem cells that will be implanted in an in vivo system, and the tumor characteristics which will be formed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ANDRADE, NATHALIA PAIVA; SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; RODINI, CAMILA OLIVEIRA; NUNES, FABIO DAUMAS. Cancer stem cell, cytokeratins and epithelial to mesenchymal transition markers expression in oral squamous cell carcinoma derived from ortothopic xenoimplantation of CD44(high) cells. PATHOLOGY RESEARCH AND PRACTICE, v. 213, n. 3, p. 235-244, 2017. (12/00786-1, 12/20622-3, 11/21395-8)