Immunohistochemistry, porous material implantation in rabbits and bone processing techniques

• Grant number: 13/01846-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: April 01, 2013
• End date: March 31, 2014
• Field of knowledge: Engineering - Materials and Metallurgical Engineering
• Principal Investigator: Ana Helena de Almeida Bressiani
• Grantee: Kalan Bastos Violin
• Supervisor: Kunio Ishikawa
• Host Institution: Instituto de Pesquisas Energéticas e Nucleares (IPEN). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São Paulo , SP, Brazil
• Institution abroad: Kyushu University, Japan
• Associated to the scholarship: 10/20698-4 - Osteointegration evaluation of metallic and ceramic implants by multiple techniques, BP.DR

Abstract

The development of biomaterials for implantation is widely performed due to the demand of them towards numbers of bone diseases, which require surgical intervention, either on orthopedics or odontological field. To replace bone tissue and restore its physiological functions is necessary that controlled physical and chemical characteristics of these implants, become favorable to the bone tissue. The interaction between cell and biomaterial is orchestrated by the proteins and membrane glycoproteins, present in bone and blood cells. Besides proteins and substances present in serum, determining the success or unsuccess of the biomaterial in contact with the tissue, as the interactions between these elements takes place at the living tissue. Thus, molecular markers, as expressed proteins by bone, can identify the biomaterials for implantation and its processes which are favorable to bone cells. The obtained porous biomaterials by our research group are: commercially pure titanium (cpTi) and the Ti-13Nb-13Zr alloy obtained by powder metallurgy, and the biphasic calcium phosphates, hydroxyapatite (HAp) and beta-tricalcium phosphate (beta-TCP). The identification of protein expression at implanted tissue will be performed by the immunohistochemistry (IHC) technique, which marks proteins in tissue slides sections properly processed. The IHC analysis of proteins that stimulate bone growth, as BMPs, osteocalcin and osteopontin, will indicate, together with the other histological molecular analysis developed, as lectinhistochemistry, the bone implanted biomaterials and its obtaining processes, which better represents results of osseointegration. (AU)