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Epigenetic evaluation of NKX3.1 and CDH1 and immunohistochemistry expression of c-Myc, NKX3.1 and E-cadherin on tissue microarray (TMA) of pre-neoplastic and neoplastic prostate of dogs

Grant number: 12/18426-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2013
Effective date (End): June 30, 2016
Field of knowledge:Biological Sciences - Morphology
Principal Investigator:Renee Laufer Amorim
Grantee:Carlos Eduardo Fonseca Alves
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated scholarship(s):14/25583-1 - Molecular prognostic markers in canine mast cell tumours, BE.EP.DR

Abstract

The canine prostate can be used as a model for a comparative study of prostate disease in humans and is the only species which occur spontaneously in the prostatic adenocarcinoma, prostatic intraepithelial neoplasia, and benign prostatic hyperplasia. The changes involved in the development of preneoplastic lesions may also play a role in tumor progression, it is important to characterize these lesions. The study will be performed on tissue microarray platforms (TMA) containing samples from different canine prostatic lesions to be assessed by immunohistochemistry. We intend to characterize this lesion in dogs using some biomarkers used in human medicine. Our research aims to identify the relationship between the expression of C-MYC protein, NKX3.1 and E-cadherin in pre-neoplastic and neoplastic canine prostate, in order to understand their role in the process of carcinogenesis. The epigenetic silencing of NKX3.1 and CDH1 is involved in prostate carcinogenesis in humans through hypermethylation. These abnormal patterns of methylation are important because they represent a change reversible, providing an important therapeutic target in human medicine. This study proposes to evaluate the methylation pattern of these genes in canine prostatic carcinoma, in order to conduct a comparative study with the methylation patterns found in human prostatic carcinoma.

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; LEIS-FILHO, ANTONIO FERNANDO; LAINETTI, PATRICIA DE FARIA; GRIECO, VALERIA; KUASNE, HELLEN; ROGATTO, SILVIA REGINA; LAUFER-AMORIM, RENEE. E-Cadherin Downregulation is Mediated by Promoter Methylation in Canine Prostate Cancer. FRONTIERS IN GENETICS, v. 10, NOV 29 2019. Web of Science Citations: 0.
FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; RIVERA CALDERON, LUIS GABRIEL; FELISBINO, SERGIO LUIS; RINALDI, JAQUELINE DE CARVALHO; DRIGO, SANDRA APARECIDA; ROGATTO, SILVIA REGINA; LAUFER-AMORIM, RENEE. Immunohistochemical panel to characterize canine prostate carcinomas according to aberrant p63 expression. PLoS One, v. 13, n. 6 JUN 12 2018. Web of Science Citations: 4.
FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; LAUFER-AMORIM, RENEE. Evaluation of NKX3.1 and C-MYC expression in canine prostatic cancer. Research in Veterinary Science, v. 118, p. 365-370, JUN 2018. Web of Science Citations: 6.
JARDIM, JOELSON; KOBAYASHI, PRISCILA E.; COSENTINO, PATRICIA D.; ALCARAZ, ANA; LAUFER-AMORIM, RENEE; FONSECA-ALVES, CARLOS E. Clinicopathological and immunohistochemical description of an intrapelvic hepatoid gland carcinoma in a 14-year-old Teckel dog. VETERINARY QUARTERLY, v. 38, n. 1, p. 9-13, DEC 7 2017. Web of Science Citations: 0.
FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILLA EMIKO; PALMIERI, CHIARA; LAUFER-AMORIM, RENEE. Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate. BMC Veterinary Research, v. 13, DEC 6 2017. Web of Science Citations: 8.
CICCONE ALVES, RENATA CINTRA; BATISTA, TATIANA LINARDI; LAUFER-AMORIM, RENEE; ELIAS, FABIANA; CALAZANS, SABRYNA GOUVEIA; FONSECA-ALVES, CARLOS EDUARDO. Clinicopathological and immunohistochemical evaluation of oesophageal leiomyosarcoma in a dog. Ciência Rural, v. 45, n. 9, p. 1644-1647, SEP 2015. Web of Science Citations: 1.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALVES, Carlos Eduardo Fonseca. Avaliação epigenética dos genes NKX3.1 E CDH1 e expressão do C-MYC, NKX3.1 e E-Caderina por imuno-histoquímica em microarranjo de tecido (TMA) de lesões pré-neoplásicas e neoplásicas na próstata de cães. 2016. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Medicina Veterinária e Zootecnia..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.