Epigenetic evaluation of NKX3.1 and CDH1 and immunohistochemistry expression of C-MYC, NKX3.1 and E-cadherin on tissue microarray (TMA) of pre-neoplastic and neoplastic prostate of dogs

• Grant number: 12/18426-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2013
• End date: June 30, 2016
• Field of knowledge: Biological Sciences - Morphology
• Principal Investigator: Renee Laufer Amorim
• Grantee: Carlos Eduardo Fonseca Alves
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 14/25583-1 - Molecular prognostic markers in canine mast cell tumours, BE.EP.DR

Abstract

The canine prostate can be used as a model for a comparative study of prostate disease in humans and is the only species which occur spontaneously in the prostatic adenocarcinoma, prostatic intraepithelial neoplasia, and benign prostatic hyperplasia. The changes involved in the development of preneoplastic lesions may also play a role in tumor progression, it is important to characterize these lesions. The study will be performed on tissue microarray platforms (TMA) containing samples from different canine prostatic lesions to be assessed by immunohistochemistry. We intend to characterize this lesion in dogs using some biomarkers used in human medicine. Our research aims to identify the relationship between the expression of C-MYC protein, NKX3.1 and E-cadherin in pre-neoplastic and neoplastic canine prostate, in order to understand their role in the process of carcinogenesis. The epigenetic silencing of NKX3.1 and CDH1 is involved in prostate carcinogenesis in humans through hypermethylation. These abnormal patterns of methylation are important because they represent a change reversible, providing an important therapeutic target in human medicine. This study proposes to evaluate the methylation pattern of these genes in canine prostatic carcinoma, in order to conduct a comparative study with the methylation patterns found in human prostatic carcinoma. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; LAUFER-AMORIM, RENEE. Evaluation of NKX3.1 and C-MYC expression in canine prostatic cancer. Research in Veterinary Science, v. 118, p. 365-370, JUN 2018. (12/16068-0, 12/18426-1, 10/13774-6)

JARDIM, JOELSON; KOBAYASHI, PRISCILA E.; COSENTINO, PATRICIA D.; ALCARAZ, ANA; LAUFER-AMORIM, RENEE; FONSECA-ALVES, CARLOS E.. Clinicopathological and immunohistochemical description of an intrapelvic hepatoid gland carcinoma in a 14-year-old Teckel dog. VETERINARY QUARTERLY, v. 38, n. 1, p. 9-13, DEC 7 2017. (12/18426-1)

DE GODOY FERNANDES, GIOVANA; PEDRINA, BRUNA; DE FARIA LAINETTI, PATRICIA; KOBAYASHI, PRISCILA EMIKO; GOVONI, VERONICA MOLLICA; PALMIERI, CHIARA; DE MOURA, VERIDIANA MARIA BRIANEZI DIGNANI; LAUFER-AMORIM, RENEE; FONSECA-ALVES, CARLOS EDUARDO. Morphological and Molecular Characterization of Proliferative Inflammatory Atrophy in Canine Prostatic Samples. CANCERS, v. 13, n. 8, p. 17-pg., 2021-04-01. (12/18426-1, 19/00497-9)

DE GODOY FERNANDES, GIOVANA; PEDRINA, BRUNA; DE FARIA LAINETTI, PATRICIA; KOBAYASHI, PRISCILA EMIKO; GOVONI, VERONICA MOLLICA; PALMIERI, CHIARA; DE MOURA, VERIDIANA MARIA BRIANEZI DIGNANI; LAUFER-AMORIM, RENEE; FONSECA-ALVES, CARLOS EDUARDO. orphological and Molecular Characterization of Proliferative Inflammatory Atrophy in Canine Prostatic Sample. CANCERS, v. 13, n. 8, APR 2021. (12/18426-1, 19/00497-9)

FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; LEIS-FILHO, ANTONIO FERNANDO; LAINETTI, PATRICIA DE FARIA; GRIECO, VALERIA; KUASNE, HELLEN; ROGATTO, SILVIA REGINA; LAUFER-AMORIM, RENEE. E-Cadherin Downregulation is Mediated by Promoter Methylation in Canine Prostate Cancer. FRONTIERS IN GENETICS, v. 10, NOV 29 2019. (12/18426-1)

CICCONE ALVES, RENATA CINTRA; BATISTA, TATIANA LINARDI; LAUFER-AMORIM, RENEE; ELIAS, FABIANA; CALAZANS, SABRYNA GOUVEIA; FONSECA-ALVES, CARLOS EDUARDO. Aspectos clínico-patológicos e imuno-histoquímicos de um caso de leiomiossarcoma esofágico canino. Ciência Rural, v. 45, n. 9, p. 1644-1647, SEP 2015. (12/18426-1)

FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILLA EMIKO; PALMIERI, CHIARA; LAUFER-AMORIM, RENEE. Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate. BMC Veterinary Research, v. 13, DEC 6 2017. (12/16068-0, 12/18426-1)

FONSECA-ALVES, CARLOS EDUARDO; KOBAYASHI, PRISCILA EMIKO; RIVERA CALDERON, LUIS GABRIEL; FELISBINO, SERGIO LUIS; RINALDI, JAQUELINE DE CARVALHO; DRIGO, SANDRA APARECIDA; ROGATTO, SILVIA REGINA; LAUFER-AMORIM, RENEE. Immunohistochemical panel to characterize canine prostate carcinomas according to aberrant p63 expression. PLoS One, v. 13, n. 6, JUN 12 2018. (12/16068-0, 12/18426-1, 10/13774-6)

BUZATTO, ANNELIESE BAETZ; ELIAS, FABIANA; FRANZONI, MAYARA SIMAO; FONSECA-ALVES, CARLOS EDUARDO. Long-Term Survival of a Cat with Primary Leiomyosarcoma of the Urinary Bladder. VETERINARY SCIENCES, v. 6, n. 3, p. 7-pg., 2019-09-01. (12/18426-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Carlos Eduardo Fonseca. Avaliação epigenética dos genes NKX3.1 E CDH1 e expressão do C-MYC, NKX3.1 e E-Caderina por imuno-histoquímica em microarranjo de tecido (TMA) de lesões pré-neoplásicas e neoplásicas na próstata de cães. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.