Internal standard addition method using flowing gradient concentration in spectroscopic techniques

• Grant number: 12/23323-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2013
• End date: February 28, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: José Anchieta Gomes Neto
• Grantee: Felipe Manfroi Fortunato
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

A new calibration method in analytical chemistry combining the standard addition and internal standardization methods is proposed in this Doctoral Thesis. The internal standard addition using gradient flow analysis is a novel method and benefits in the field of quantitative chemical analysis are: a) correct for matrix or transport effects, and fluctuations due to changes in sample size, instrumental parameters; b) minimal manipulation of sample and standard solutions, c) reduction of reagent consumption and analysis time; d) minimization of contamination and loss of analyte by employing dynamical systems in pipelines; e) analyst exposure (or contact) to numerous chemicals is reduced. The benefits of this proposal are clear and will be demonstrated by using two spectroscopic techniques: high-resolution continuum source flame atomic absorption spectrometry (in the determination of Ca, K, Mg and Na in biodiesel and urine) and Raman spectroscopy (in the determination of uric acid, creatinine and urea in urine; benzene, toluene, ethylbenzene and xylenes - BTEX - in gasoline, methanol in gasoline, ethanol and biodiesel, biodiesel in diesel).

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIRANDA, KELBER; VIEIRA, ALAN LIMA; BECHLIN, MARCOS ANDRE; FORTUNATO, FELIPE MANFROI; VIRGILIO, ALEX; FERREIRA, EDILENE CRISTINA; GOMES NETO, JOSE ANCHIETA. Determination of Ca, Cd, Cu, Fe, K, Mg, Mn, Mo, Na, Se, and Zn in Foodstuffs by Atomic Spectrometry After Sample Preparation Using a Low-Cost Closed-Vessel Conductively Heated Digestion System. FOOD ANALYTICAL METHODS, v. 9, n. 7, p. 1887-1894, JUL 2016. (14/18393-1, 12/23323-7, 14/12595-1, 12/02891-7)

FORTUNATO, FELIPE MANFROI; BECHLIN, MARCOS ANDRE; FERREIRA, EDILENE CRISTINA; OLIVEIRA, SILVANA RUELLA; GOMES NETO, JOSE ANCHIETA. Evaluation of Nitrate as Internal Standard for Quantitative Determination of Urea in Urine by Raman Spectroscopy. BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY, v. 5, n. 19, p. 22-28, APR-JUN 2018. (12/23323-7)

FORTUNATO, FELIPE M.; BECHLIN, MARCOS A.; GOMES NETO, JOSE A.; VIRGILIO, ALEX; DONATI, GEORGE L.; JONES, BRADLEY T.. Standard dilution analysis in flow system: Sodium determination by flame atomic emission spectrometry. Microchemical Journal, v. 124, p. 662-667, JAN 2016. (14/18393-1, 14/12595-1, 12/23323-7)

FORTUNATO, FELIPE MANFROI; BECHLIN, MARCOS A.; GOMES NETO, JOSE A.; DONATI, GEORGE L.; JONES, BRADLEY T.. Internal standard addition calibration: Determination of calcium and magnesium by atomic absorption spectrometry. Microchemical Journal, v. 122, p. 63-69, SEP 2015. (12/23323-7)

BECHLIN, MARCOS ANDRE; FORTUNATO, FELIPE MANFROI; DA SILVA, RICARDO MOUTINHO; FERREIRA, EDILENE CRISTINA; GOMES NETO, JOSE ANCHIETA. A simple and fast method for assessment of the nitrogen-phosphorus-potassium rating of fertilizers using high-resolution continuum source atomic and molecular absorption spectrometry. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, v. 101, p. 240-244, NOV 1 2014. (12/13500-9, 12/23323-7)

BECHLIN, MARCOS A.; FORTUNATO, FELIPE M.; FERREIRA, EDILENE C.; GOMES NETO, JOSE A.; NOBREGA, JOAQUIM A.; DONATI, GEORGE L.; JONES, BRADLEY T.. Bismuth as a general internal standard for lead in atomic absorption spectrometry. Analytica Chimica Acta, v. 831, p. 24-30, JUN 11 2014. (12/13500-9, 12/23323-7)

FORTUNATO, FELIPE M.; VIEIRA, ALAN L.; GOMES NETO, JOSE A.; DONATI, GEORGE L.; JONES, BRADLEY T.. Expanding the potentialities of standard dilution analysis: Determination of ethanol in gasoline by Raman spectroscopy. Microchemical Journal, v. 133, p. 76-80, JUL 2017. (14/12595-1, 12/23323-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FORTUNATO, Felipe Manfroi. Evaluation of the internal standard addition method in spectroscopic techniques. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.