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Cloning and heterologous expression of epoxide hydrolase enzime of Candida albicans in Escherichia coli.

Grant number: 12/25498-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2013
Effective date (End): August 31, 2013
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal researcher:Anete Pereira de Souza
Grantee:Raphael Pedro Ricci
Home Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

This project aims cloning, expression and initial characterization of an epoxide hydrolase (EH) of the yeast Candida albicans CCT 0776. The EH activity of this yeast has been detected and studied by the research group of Professor. Anita J. Marsaioli (Institute of Chemistry - IQ / Unicamp) that motivated this study. Therefore, searches were performed at NCBI sequences of EH and the genus Candida. Based on homology between them, pairs of primers were designed to amplify the gene EH. Gene amplification by PCR enables nucleotide sequencing, cloning, expression and purification of the recombinant enzyme. Therefore, the project that follows details the planning for the study of this important enzyme widespread use of biotechnology.