The role of VEGF and dentin-derived factors in the differentiation of stem cells from human exfoliated teeth (shed) in odontoblasts and endothelial cells

• Grant number: 12/24244-3
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: July 01, 2013
• End date: June 30, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Carlos Henrique Ribeiro Camargo
• Grantee: Gleyce Oliveira Silva
• Supervisor: Jacques E. Nör
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
• Institution abroad: University of Michigan, United States
• Associated to the scholarship: 12/13039-0 - LRP6 AND FRIZZLED6 EFFECTS IN THE ENDOTHELIAL DIFFERENTIATION OF DPSC, BP.DR

Abstract

The aim of this study is test the hypothesis that stem cells from exfoliated human teeth (SHED) may have its odontoblastic and endothelial differentiation enhanced by the association of VEGF and dentin-derived factors and confirm that these cells are true. Tooth slices are obtained from freshly extracted third molars for the use of dentin-derived factors. SHED will be seeded on tooth slices, treated or not treated with EDTA, composed of PLLA matrix and treated or not treated with VEGF, and then implanted into the back of immunosuppressed rats. The sample will be collected and send to a microdissection laser capture (MCL) microscope to gene expression of VE-Cadherin, CD31, DSPP and DMP-1, as markers of differentiation processes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, GLEYCE O.; ZHANG, ZHAOCHENG; CUCCO, CAROLINA; OH, MIN; CAMARGO, CARLOS H. R.; NOER, JACQUES E.. Lipoprotein Receptor-related Protein 6 Signaling is Necessary for Vasculogenic Differentiation of Human Dental Pulp Stem Cells. JOURNAL OF ENDODONTICS, v. 43, n. 9, p. 6-pg., 2017-09-01. (12/13039-0, 12/24244-3)

SILVA, GLEYCE O.; ZHANG, ZHAOCHENG; CUCCO, CAROLINA; OH, MIN; CAMARGO, CARLOS H. R.; NOER, JACQUES E.. Lipoprotein Receptor-related Protein 6 Signaling is Necessary for Vasculogenic Differentiation of Human Dental Pulp Stem Cells. JOURNAL OF ENDODONTICS, v. 43, n. 9, S, p. S25-S30, SEP 2017. (12/13039-0, 12/24244-3)