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Ultra-weak light emission in protozoa

Grant number: 13/01501-3
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Start date: June 03, 2013
End date: September 02, 2013
Field of knowledge:Agronomical Sciences - Agricultural Engineering - Soil and Water Engineering
Principal Investigator:José Euclides Stipp Paterniani
Grantee:Samuel Ricardo dos Santos
Supervisor: Masakazu Katsumata
Host Institution: Faculdade de Engenharia Agrícola (FEAGRI). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Institution abroad: Hamamatsu Photonics K. K., Japan  
Associated to the scholarship:10/07792-1 - ULTRAWEAK LIGHT EMISSION DETECTION IN Giardia spp, BP.DR

Abstract

It is proposed the characterization of a new screening experiment for protozoa samples (Giardia lamblia and Cryptosporidium parvum) using chemical probes and delayed fluorescence (DF) measurements, in portable devices able to work in the photon-count mode. The main goal is to distinguish the living from the dead (oo)cysts, when arranged in concentrated samples, and to compare with the results recently obtained at UNICAMP in a current PhD project.Both series of experiments (Giardia and Cryptosporidium) will be designed at Hamamatsu K. K. Labs, with pre and post data analyzes as well in collaboration with Dr. Katsumata's group. Experiments with Giardia and Cryptosporidium samples will be properly held at NIID, Tokyo, with support of Hamamatsu's experimental setups and NIID expertise's. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, SAMUEL RICARDO; BRANCO, NILSON; BUENO FRANCO, REGINA MAURA; STIPP PATERNIANI, JOSE EUCLIDES; KATSUMATA, MASAKAZU; BARLOW, PETER W.; GALLEP, CRISTIANO DE MELLO. Fluorescence decay of dyed protozoa: differences between stressed and non-stressed cysts. LUMINESCENCE, v. 30, n. 7, p. 1139-1147, . (10/07792-1, 11/10763-6, 13/01501-3)