Genetic structure of Epidendrum secundum (Orchidaceae) based on nuclear genetic markers

• Grant number: 13/02453-2
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: April 15, 2013
• End date: June 14, 2013
• Field of knowledge: Biological Sciences - Botany - Plant Taxonomy
• Principal Investigator: Fábio de Barros
• Grantee: Fábio Pinheiro
• Supervisor: Salvatore Cozzolino
• Host Institution: Instituto de Botânica. Secretaria de Meio Ambiente, Infraestrutura e Logística (São Paulo - Estado). São Paulo , SP, Brazil
• Institution abroad: Università degli Studi di Napoli Frederico II (UNINA), Italy
• Associated to the scholarship: 09/15052-0 - Phylogeography of the species of Epidendrum (Orchidaceae) belonging to the Atlantic clade (subgenus Amphiglottium), BP.PD

Abstract

The genetic structure of natural populations can reveal interesting patterns regarding the evolution and early stages of speciation. The extensive amount of molecular markers available offers an interesting opportunity to investigate the evolution of early stages of speciation, mainly in natural populations. The main objective of this study is to apply the AFLP (amplified fragment length polymorphisms) technique to analyze the genetic differentiation patterns of natural populations of Epidendrum secundum (Orchidaceae), including its geographic distribution along Central Brazilian Range and Atlantic Forest. Approximately 350 individuals sampled on 16 populations will be analyzed using ten different primer combinations of AFLP markers. Bayesian assignment analysis will be applied in order to identify major genetic clusters. Gene flow patterns will be estimated in order to investigate differential gene flow among loci, using the software INTROGRESS. The early stages of speciation and the existence of incipient species will be investigated using the plastid sequences dataset which will be available, as described in the main postdoc project. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINHEIRO, FABIO; DE MELO E GOUVEIA, TIAGO MANUEL ZANFRA; COZZOLINO, SALVATORE; CAFASSO, DONATA; CARDOSO-GUSTAVSON, POLIANA; SUZUKI, ROGERIO MAMORU; PALMA-SILVA, CLARISSE. Strong but permeable barriers to gene exchange between sister species of Epidendrum. AMERICAN JOURNAL OF BOTANY, v. 103, n. 8, p. 1472-1482, AUG 2016. (09/15052-0, 10/19617-0, 09/52725-3, 13/02453-2)

PINHEIRO, FABIO; CAFASSO, DONATA; COZZOLINO, SALVATORE; SCOPECE, GIOVANNI. Transitions between self-compatibility and self-incompatibility and the evolution of reproductive isolation in the large and diverse tropical genus Dendrobium (Orchidaceae). ANNALS OF BOTANY, v. 116, n. 3, p. 457-467, SEP 2015. (09/15052-0, 13/02453-2)