Comparison between the colonies forming units on ocular surface, before and after antisepsis ocular surface with solutions of povidone-iodine at 1% and 2% and 0.9% saline by two treatments: treatment 1

• Grant number: 13/00456-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2013
• End date: December 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Cristiane dos Santos Honsho
• Grantee: Camila Caceres Ferracini
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Postoperative ocular infections, despite the low occurrence, are potentially serious considering its great potential to lead to loss of vision in a short time. Different techniques of antisepsis ocular surface may be adopted in order to minimize the risks of post-surgical infections, however meager records are available asserting the effectiveness of such techniques. Thus, two methods are proposed for antisepsis ocular surface (Treatments 1 and 2), with solutions of povidone-iodine at 1% and 2% and saline solution at 0.9%. In Treatment 1, it will accomplish the ocular surface antisepsis with 10 mL of the solutions by administering them as a bolus, as GI50 groups, and GI100 GSS, respectively. In GI50 (n = 6), antisepsis is performed with 10 ml of 1% povidone iodine applied to the ocular surface as a bolus. In GI100 (n = 6), will proceed to conduct with the same solution at 2% and GSS (n = 6), use will saline solution at 0.9%. In treatment 2, the antiseptic will be made with the same solutions, administering the volume of 3ml in the form of drip (1 drop/second), composing the groups GI50g (n = 6), GI100g (n = 6) and GSSG (n = 6). The solutions are prepared at the time of use. Samples are collected from the surface with sterile swab, immediately before atissepsia (T0), after two minutes (T2), thirty minutes (T30) and sixty minutes (T60). The samples are transferred to the microbiology laboratory in a test tube with phosphate buffer and processed for microbiological culture within two hours after collection. After processing and incubation time, colonies on the plates that will be present from 30 to 300 colonies, and the results are expressed in CFU/mL. The results will be statistically analyzed. (AU)