|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||June 01, 2013|
|Effective date (End):||September 30, 2014|
|Field of knowledge:||Biological Sciences - Botany - Pant Physiology|
|Principal Investigator:||Antonio Vargas de Oliveira Figueira|
|Grantee:||Luís Henrique Damasceno Serezino|
|Home Institution:||Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil|
The intensification of the modern agriculture, coupled with high demand for renewable energy sources has led to the indiscriminate use of nitrogenous fertilizers which may cause a massive environmental impact. Therefore, crops with improved nitrogen use efficiency are needed. In Brazil, sugarcane has great economical importance and the growing cultivated area requires a high nitrogen input in order to increase yield. Compared to other crop species, the nitrate use efficiency by sugarcane is low however the genetic and physiological causes of this reduced response remains unknown. The objective of this project is to characterize the process of nitrate uptake in sugarcane roots in order to identify its regulation in relation to plant N status or according to supply inorganic N sources (ammonium and/or nitrate). Further, characterize the transport and remobilization of shoot nitrate through uptake kinetic studies and 15N-nitrate re-allocation experiments. The profile of transcriptional gene family NITRATE/ PEPTIDE TRANSPORTER (NRT / PRT) the nitrate membrane transporters in plants will be characterize and correlated to the uptake and reallocation of nitrate in sugarcane.