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Physiological characterization and gene expression profile of transportation during acquisition and nitrate remobilization in sugarcane (Saccharum spp.)

Grant number: 13/02702-2
Support Opportunities:Scholarships in Brazil - Master
Start date: June 01, 2013
End date: September 30, 2014
Field of knowledge:Biological Sciences - Botany - Pant Physiology
Principal Investigator:Antonio Vargas de Oliveira Figueira
Grantee:Luís Henrique Damasceno Serezino
Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

The intensification of the modern agriculture, coupled with high demand for renewable energy sources has led to the indiscriminate use of nitrogenous fertilizers which may cause a massive environmental impact. Therefore, crops with improved nitrogen use efficiency are needed. In Brazil, sugarcane has great economical importance and the growing cultivated area requires a high nitrogen input in order to increase yield. Compared to other crop species, the nitrate use efficiency by sugarcane is low however the genetic and physiological causes of this reduced response remains unknown. The objective of this project is to characterize the process of nitrate uptake in sugarcane roots in order to identify its regulation in relation to plant N status or according to supply inorganic N sources (ammonium and/or nitrate). Further, characterize the transport and remobilization of shoot nitrate through uptake kinetic studies and 15N-nitrate re-allocation experiments. The profile of transcriptional gene family NITRATE/PEPTIDE TRANSPORTER (NRT/PRT) the nitrate membrane transporters in plants will be characterize and correlated to the uptake and reallocation of nitrate in sugarcane. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA, JONI E.; SEREZINO, LUIS H. D.; ALVES, MELISSA K.; TAGLIAFERRO, ANDRE L.; VITTI, MARIELLE; CRESTE, SILVANA; RIANO-PACHON, DIEGO M.; DOS SANTOS, RENATO, V; FIGUEIRA, ANTONIO. Root nitrate uptake in sugarcane (Saccharum spp.) is modulated by transcriptional and presumably posttranscriptional regulation of the NRT2.1/NRT3.1 transport system. Molecular Genetics and Genomics, v. 297, n. 5, p. 19-pg., . (13/15989-8, 13/02702-2, 10/11313-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SEREZINO, Luís Henrique Damasceno. Physiological and transcriptional characterization of the process of nitrate uptake and remobilization in sugarcane (Saccharum spp.). 2015. Master's Dissertation - Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB) Piracicaba.