Clinical, laboratory and histopathological evaluation of urinary system of feedlot sheep with and without supplementation with ammonium chloride

• Grant number: 12/24985-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2013
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Roberto Calderon Gonçalves
• Grantee: Bianca Paola Santarosa
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The obstructive urolithiasis has a high incidence in sheep. The urinary acidification with ammonium chloride is an effective method for preventing the disease. This study aims to determine if the diet provided to the animals in confinement, rich in concentrate, causes lesions in the urinary system of sheep, and to analyze the difference of the histopathological features of the urinary system between animals that will receive ammonium chloride and those that will not. 60 male sheep of defined breed will be used, aged from three to six months, randomly divided into two groups: Group I (n = 40) - that will receive 10g of ammonium chloride/animal/day orally for 42 consecutive days, Group II (n = 20) - control animals not treated with ammonium chloride. The diet will consist of 85% concentrate and 15% hay, and water ad libitum. For safety trial, mineral and chemical analyzes of the total ration will be held. Moments (M) of clinical evaluation and blood collection for determination of serum urea and creatinine: M0 - just before the start of the experiment and at intervals of seven days at M1, M2, M3, M4, M5 and M6. The animals will be observed daily and the incidence of urolithiasis in both groups will be recorded throughout the experimental period. After the period of confinement, the animals will be slaughtered in a slaughterhouse inspected, and the urinary system will be collected for macro and microscopic examination. If the animal comes to death even with treatment, necropsy will be performed promptly, and the urinary system (kidneys, ureters, bladder and penile urethra) will be collected and processed for histopathological examination. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTAROSA, Bianca Paola. Clinical, laboratory and anatomopathological evaluation of the urinary system of feedlot sheep wit and without ammonium chloride supplementation. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.