Analysis the lipid profile of sperm membrane from stallions with high and low resistance to freezing semen

• Grant number: 12/24843-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2013
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marco Antonio Alvarenga
• Grantee: Carlos Ramires Neto
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The equine semen freezing enables storage the genetic material of stallions for an unlimited time and mate animals geographically distant. However, the semen of some stallions, especially Mangalarga Marchador breed, has inadequate quality after thawing for use in artificial insemination. Some authors have described the composition and proportion of the lipids that make up the plasma membrane of the sperm interferes with the cell resistance to damage from sperm cryopreservation. This way, this study aims to to analyze the lipid profile of the plasmatic membrane of the sperm of stallions and determine their relationships with their resistance to freezing sperm. In addition, the objective is to check for differences in these lipid components of the plasmatic membrane of sperm from different breeds stallion. For this will be used 40 stallions, 20 animals (10 Quarter Horse and 10 Mangalarga Marchador) with semen with good resistance to freezing sperm and 20 stallions (10 Quarter Horse and 10 Mangalarga Marchador) with sperm susceptible to damage due to freezing. The semen will be collected in an artificial vagina and kinetic analysis will be performed and sperm plasmatic membrane integrity in semen before and after freezing. Analysis the lipid profile of sperm plasmatic membrane in the fresh semen will be made using the technique of mass spectrometry MALDI-TOF.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RAMIRES NETO, Carlos. Perfil lipídico da membrana plasmática e alterações morfofuncionais de esperamatozóides de garanhões resistentes e sensíveis à criopreservação. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.