Evaluation of the antioxidant effects of sucralfate in the colonic mucosa of intestinal transit exclusion: an experimental study in rats

• Grant number: 12/16236-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2013
• End date: April 30, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Carlos Augusto Real Martinez
• Grantee: Giovanna Riccitelli do Couto
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil

Abstract

Diversion colitis (DC) is a disease characterized by the development of chronic inflammation in the mucous layer of segments of the colon when they were deprived of the intestinal stream. The disease presents clinical, endoscope, and histopathological aspects, similar to inflammatory bowel diseases suggesting a common etiopathogenic basis. The DC is related to intraluminal deficiency of short-chain fatty acids (SCFA), which are the main energy substrate for the cells of the colon epithelium. The intraluminal deficiency of the SCFA alters the energetic metabolism of the colic epithelial cells, leading to the formation of reactive oxygen species that determine injury of the different mechanisms of defense of the epithelial colic barrier represented by the layer of mucus, cellular membrane, proteins of cellular adhesion and basal membrane, allowing to the invasion of the sterile submucosa layer for bacteria existent found in the intestinal light, allowing to migration of leucocytes that they characterize the disease. Although the DC is a relatively common disease and with the incidence in progression, where the oxidative stress represents the etiopathogenic basis, until the present date the effects of natural substances with antioxidant effects were not studied experimentally such as 5-aminosalicylic acid, n-acetylcysteine, sucralfate, the aqueous extracts of Ilex paraguariensis and Curcuma longa in experimental models of DC. Likewise, the effect of these substances is still not explained in the prevention of the oxidative stress to the different mechanisms that form the epithelial barrier of the colic mucosa, as well as expression of the inflammatory mediators' substances in animals undergone to the DC. The aim of the present study is to value, experimentally, the antioxidant effects of the 5-aminosalicylic acid, n-acetylcysteine, sucralfate, aqueous extract of the Ilex paraguariensis and Curcuma longa in the prevention of the tissue oxidative stress, in the expression of proteins wrapped in the integrity of the epithelial barrier of the colic mucosa and in the expression of inflammatory mediators in the colon of rats submitted to diversion of the fecal stream. (AU)