Pluripotency genes in Zebrafish (Danio rerio) testis: As means to identify, candidate markers for spermatogonial stem cells

• Grant number: 13/03384-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2013
• End date: December 19, 2015
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Principal Investigator: Rafael Henrique Nóbrega
• Grantee: Lucas Benites Doretto
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 14/18325-6 - Characterization of vasa and its promoter region in jundiá (Rhamdia quelen) to produce a transgenic fish line expressing green fluorescent protein under the control of the vasa promoter, BE.EP.IC

Abstract

Stem cells are classified into two broader types based on their embryological origin and ability to differentiate. Embryonic stem cells (ESCs) are originated from the zygote; considered totipotent which can give rise to the whole organism or pluripotent which can originate from the three germinal layers - ecto, meso-endoderm. Adult stem cells (ASCs) are the stem cells found in the fetal and adult tissue; classified as uni, oligo, or multipotent according to the variety of adult tissues derived from them. The interest of ESCs is noted worth in regenerative medicine because of their ability to be propagated and differentiated into different cell types. However, restrictions still exist on their use due to ethical conflicts. ASCs are free of restrictions and they're in vitro differentiation has been started to be investigated by several groups of research. Pluripotent markers, such as surface antigens, telomerase, and genes like OCT4 and NANOG are expressed in ESCs and some ASCs, and are suppressed when stem cells differentiate. The aim of the current project is to investigate pluripotent genes in the adult zebrafish testes as a means to identify candidate markers for spermatogonial stem cells. The use of these markers to select and enrich spermatogonial stem cells before transplantation is necessary not only to increase the efficiency of germ cell transplantation but also in the biotechnology applied to aquaculture and to better understand the stem cell biology in fish. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, MARCOS A.; MARTINEZ, EMANUEL R. M.; BUTZGE, ARNO J.; DORETTO, LUCAS B.; RICCI, JULIANA M. B.; RODRIGUES, MAIRA S.; VIGOYA, ANGEL A. A.; GOMEZ-GONZALEZ, NURIA E.; STEWART, AMANDA B.; NOBREGA, RAFAEL H.. Molecular characterization and expression analysis of anti-Mullerian hormone in common carp (Cyprinus carpio) adult testes. GENE EXPRESSION PATTERNS, v. 40, JUN 2021. (13/03384-4, 14/25313-4, 12/00423-6, 19/02950-2)