Evaluation of the effects of hypertonic saline solution on mesenteric microcirculation response in Escherichia coli-induced sepsis model in rats

• Grant number: 13/04135-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2013
• End date: April 30, 2014
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Paulina Sannomiya
• Grantee: Mariane Taborda
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Elder and immunossupressed patients exposed to trauma and hemorrhage are more prone to develop sepsis, septic shock, and systemic inflammatory response syndrome. Early goal directed therapy is critical for tissue perfusion associated to systemic microcirculatory dysfunction. Experimental evidences show that small volumes of hypertonic saline solution (7.5%) induce blood volume expansion, restore cardiac output, and regional blood flow. This study aims to investigate the effects of hypertonic saline solution treatment on the mesenteric microcirculation in an E.coli-induced sepsis model in rats. Experiments include the evaluation of the expression of endothelial cell adhesion molecules, P-selectin and ICAM-1, by immunohistochemistry, and leukocyte-endothelial interactions at mesenteric microcirculation, by intravital microscopy.