Characterization of tauroursodesoxicolic (TUDCA) action on the pancreatic endocrine function and action in tissue glucagon target ob / ob mice

• Grant number: 13/01318-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2013
• End date: August 31, 2017
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Everardo Magalhães Carneiro
• Grantee: Jean Franciesco Vettorazzi
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 16/07127-4 - TUDCA modulates pancreatic alpha cell and glucagon secretion, BE.EP.DR

Abstract

Tauroursodeoxycholic acid (TUDCA) is formed from the conjugation of bile acid ursodeoxycholic (UDCA) with the amino acid taurine (Tau). Besides the well-described actions on gastrointestinal function, the TUDCA contributes to the restoration of glucose homeostasis in Type 2 Diabetes Mellitus (T2DM), regulation of energy metabolism and adiposity. Literature data indicate the action of TUDCA as chaperone molecule mechanism against endoplasmic reticulum stress (ER) that cause cell damage contributing to the pathophysiology of obesity and T2DM. Most studies attempted to demonstrate the action of TUDCA on insulin target tissues (liver, hypothalamus, brown adipose tissue) but the action of this compound on pancreatic endocrine morphofunction is not known, although different studies showed that bile acid can activate kinases such as protein kinase (PK) and C-mitogen activated kinase (MAPK) and regulate movement of Ca2 +. Thus, this project aims to characterize the effect of TUDCA and its mechanism of action on the secretion of insulin and glucagon in pancreatic islets isolated from mice. Also, check glucose homeostasis, pancreatic and morphofunction action of glucagon in ob / ob mice treated with TUDCA. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VETTORAZZI, JEAN FRANCIESCO; KURAUTI, MIRIAN AYUMI; SOARES, GABRIELA MOREIRA; BORCK, PATRICIA CRISTINE; FERREIRA, SANDRA MARA; SOUTO BRANCO, RENATO CHAVES; LIMA MICHELONE, LUCIANA DE SOUZA; BOSCHERO, ANTONIO CARLOS; COSTA JUNIOR, JOSE MARIA; CARNEIRO, EVERARDO MAGALHAES. Bile acid TUDCA improves insulin clearance by increasing the expression of insulin-degrading enzyme in the liver of obese mice. SCIENTIFIC REPORTS, v. 7, NOV 1 2017. (14/01717-9, 15/12611-0, 13/01318-4)

VETTORAZZI, JEAN FRANCIESCO; RIBEIRO, ROSANE APARECIDA; BORCK, PATRICIA CRISTINE; SOUTO BRANCO, RENATO CHAVES; SORIANO, SERGI; MERINO, BEATRIZ; BOSCHERO, ANTONIO CARLOS; NADAL, ANGEL; QUESADA, IVAN; CARNEIRO, EVERARDO MAGALHAES. The bile acid TUDCA increases glucose-induced insulin secretion via the cAMP/PKA pathway in pancreatic beta cells. METABOLISM-CLINICAL AND EXPERIMENTAL, v. 65, n. 3, p. 54-63, MAR 2016. (13/01318-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VETTORAZZI, Jean Franciesco. Effects of the bile acid tauroursodeoxycholic (TUDCA) in glycemic homeostasis: insulin and glucagon. 2017. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.