Rhinovirus replication in explants of human tonsillar lymphoepithelial tissue infected ex vivo

• Grant number: 13/06380-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2013
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Eurico de Arruda Neto
• Grantee: Ronaldo Bragança Martins Júnior
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Human rhinovirus (HRV), a picornavirus in the genus Enterovirus, is classified in three species: HRV-A, HRV-B and HRV-C. HRVs are viruses with icosahedral symmetry, non-enveloped, with positive-strand RNA genome of approximately 7.2 kb. HRV is responsible for the majority of acute respiratory infections (ARI), known as 'common colds', but also causes acute otitis media, acute maxillary sinusitis and asthma exacerbations. The fact that co-detections of HRV with other viruses is common, and the high frequency of detection in lymphoid tissues of adenoids and tonsils from patients without ARI symptoms, indicates the possibility of persisting/latent infections. Mechanisms involved with persistence and latency of RNA viroses are still obscure, and the present project aims at understanding such mechanism in the case of HRV. The goal is to elucidate infection pathways in tonsillar explants infected ex vivo and thus shed light on possible mechanisms of persistence. Two experimental models will be used: inoculation of explants with laboratory HRV stocks (including fluorescent HRV); and ex vivo cultures of explants of naturally infected tonsils. Replication sites will be localized and viral (+) and (-) strand RNAs will be quantitated. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PUCCA, MANUELA BERTO; CERNI, FELIPE AUGUSTO; PINHEIRO-JUNIOR, ERNESTO LOPES; ZOCCAL, KARINA FURLANI; FIGUEIREDO BORDON, KARLA DE CASTRO; AMORIM, FERNANDA GOBBI; PEIGNEUR, STEVE; VRIENS, KIM; THEVISSEN, KARIN; ANGELO CAMMUE, BRUNO PHILIPPE; et al. Non-disulfide-bridged peptides from Tityus serrulatus venom: Evidence for proline-free ACE-inhibitors. Peptides, v. 82, p. 44-51, AUG 2016. (13/21342-7, 14/03332-7, 12/13590-8, 13/06380-0, 14/07125-6, 13/21329-0, 11/12317-3)

SABINO-SANTOS, JR., GILBERTO; MOTTA MAIA, FELIPE GONCALVES; MARTINS, RONALDO BRAGANCA; GAGLIARDI, TALITA BIANCA; DE SOUZA, WILLIAM MARCIEL; MUYLAERT, RENATA LARA; DE SOUZA LUNA, LUCIANO KLEBER; MELO, DANILO MACHADO; CARDOSO, RICARDO DE SOUZA; BARBOSA, NATALIA DA SILVA; et al. Natural infection of Neotropical bats with hantavirus in Brazil. SCIENTIFIC REPORTS, v. 8, JUN 13 2018. (12/03700-0, 12/04096-0, 16/02568-2, 12/24150-9, 15/05354-0, 13/06380-0, 11/19897-5, 11/06810-9, 14/02438-6, 11/22663-6, 15/06142-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARTINS JÚNIOR, Ronaldo Bragança. Rhinovirus infection in lymphoid tissues from hypertrophic human tonsils. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.