Understanding the activity of functionalized nanoparticles against virus using coherent diffraction imaging

• Grant number: 13/12190-9
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: October 01, 2013
• End date: September 30, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Mateus Borba Cardoso
• Grantee: Juliana Martins de Souza e Silva
• Supervisor: Franz Pfeiffer
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Institution abroad: Technical University of Munich (TUM), Germany
• Associated to the scholarship: 12/05481-4 - Surface chemistry manipulation of silver-based nanocomposites: study of biocidal effect against enveloped virus, BP.PD

Abstract

Viruses are among the most harmful pathogens to the human population, being responsible for the death of millions of people each year. Vaccines therapies are not available for several diseases caused by virus, impelling the development of new strategies for their control. Nanoparticles have shown activity against a number of viruses and are promising materials for antiviral therapies. Understanding the interaction between virus and nanoparticles is crucial for the progress of these therapies. Preliminary biological tests (Project 2012/05481-4) suggest that core-shell nanoparticles made of silver core and mesoporous silica shell (AgNP-SM) functionalized with mercapto, glycidyl, isocyanate, amino or phenyl ethyl have the ability to reduce the viral infection in cells. Different degrees of viral infection reduction are observed depending on the chemical groups present on the surface of the AgNP-SM, which possibly results on different types and degrees of interaction between the nanostructures and viruses or cells. High resolution 3D imaging of the interactions between nanocomposites and virus may give information about the structural basis involved in viral infection control. Therefore, the project here described aims at understanding the interaction between virus and AgNP-SM by using coherent diffraction imaging. These images will help understanding the antiviral action of nanomaterials and will contribute to explain the antiviral activity of the functionalized core-shell nanocomposites as a function of the surface chemical groups, allowing the development of novel antiviral therapies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, JULIANA MARTINS DE S. E; ZANETTE, IRENE; NOEL, PETER B.; CARDOSO, MATEUS B.; KIMM, MELANIE A.; PFEIFFER, FRANZ. Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography. SCIENTIFIC REPORTS, v. 5, SEP 25 2015. (13/12190-9)

DE SOUZA E SILVA, JULIANA MARTINS; MELO HANCHUK, TALITA DINIZ; SANTOS, MURILO IZIDORO; KOBARG, JOERG; BAJGELMAN, MARCIO CHAIM; CARDOSO, MATEUS BORBA. Viral Inhibition Mechanism Mediated by Surface-Modified Silica Nanoparticles. ACS APPLIED MATERIALS & INTERFACES, v. 8, n. 26, p. 16564-16572, JUL 6 2016. (13/12190-9, 14/22322-2, 12/05481-4)