Evaluation system Clock in peripheral tissues of rats under different feeding patterns

• Grant number: 12/25418-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2013
• End date: June 30, 2014
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Paula Condé Lamparelli Elias
• Grantee: Jennyfer Fernanda Rodrigues Domingues
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Circadian rhythms are biological rhythms that hover around 24 hours and are essential for survival. These rhythms are controlled by external synchronizers as light and power and even persist without these environmental cues. The activities of peripheral clocks are synchronized by the central clock via humoral and neural connections that have not been fully elucidated. The demonstration that a discrete neural structure functions as a true biological clock because some external stimulus is a fundamental step towards understanding the mechanisms of timing. In principle, the elucidation of the components of the clock by anatomical-histochemical techniques was the first step in understanding the neural mechanisms involved. The use of molecular genetics techniques and lets it start to study the innermost mechanisms of the oscillator, but still understanding its operation is still far from being achieved. It is not known if there is any interference from the biological clock genes in animals subjected to different dietary patterns and what role in this organism. This project aims to evaluate the expression of genes Clock, BMAL1, Per1, Per2, Per3, Cry1, and Cry2, by quantitative PCR (qPCR) in adrenal and subcutaneous abdominal and back periepididymal tissues of rats submitted to changes in eating patterns. (AU)