Scholarship 12/22164-2 - Ontogenia, Endocrinologia - BV FAPESP
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Ontogeny of the circadian rhythm of the Hypothalamic-Pituitary-Adrenal in Wistar rats: comparison of gene expression of "Clock" system and the circadian rhythm of corticosterone.

Grant number: 12/22164-2
Support Opportunities:Scholarships in Brazil - Doctorate
Start date until: March 01, 2013
End date until: February 29, 2016
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Ayrton Custodio Moreira
Grantee:Silvia Liliana Ruiz Roa
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The biological rhythms result from the interaction of the endogenous circadian pacemaker and the environmental synchronizing agents. The clock genes and expressed proteins have been described as being responsible for creating molecular cycles associated with 24-hour environmental cycle. The activity of the Hypothalamic-Pituitary-Adrenal (HPA) shows a circadian pattern of secretion, where circulating levels of glucocorticoids fluctuate throughout the 24 hours. Conversely, the HPA axis influences the activity / circadian rhythm system "Clock", where glucocorticoids affect the peripheral clocks in almost all organs and tissues. The suprachiasmatic nucleus (SCN) presents rhythmicity in the expression of some genes of the "Clock" between the 2nd and 10th day after birth and the circadian rhythm of plasma corticosterone secretion is detected from the 16th day postnatal rats Wistar. In the adult rat's adrenal rhythmic expression of proteins involved in steroidogenesis is unknown however the postnatal age at which this starts rhythmicity is present and is synchronized with the ontogeny of circadian rhythms of gene expression system "Clock" and secretion corticosterone. In the present study will evaluate the expressions of proteins CLOCK/BMAL1, PERs, Crys, ROR, NR1D1 (Western Blotting), and the genes Clock, Bmal1, Per1, Per2, Per3, Cry1, Cry2, Rev-erb± and ROR± (PCR Real-time TaqMan Ô) in the SCN, and the StAR protein and MC2R in adrenal, whose fluctuations appear to be modulated by the circadian system "Clock". Simultaneously be determined diurnal variations in the concentrations of plasma corticosterone in rat pups at different times after birth. Differences between groups will be analyzed by nonparametric methods using the Mann-Whitney test and Fisher's exact test. Correlations are calculated by the Spearman test. The significance level is 5%.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RUIZ ROA, SILVIA LILIANA; MARTINEZ, EDSON ZANGIACOMI; MARTINS, CLARISSA SILVA; ANTONINI, SONIR RAUBER; DE CASTRO, MARGARET; MOREIRA, AYRTON CUSTODIO. Postnatal Ontogeny of the Circadian Expression of the Adrenal Clock Genes and Corticosterone Rhythm in Male Rats. Endocrinology, v. 158, n. 5, p. 1339-1346, . (12/22164-2, 13/09799-1)

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