Energy balance and body fluid homeostasis control: from cells to the physiological systems

• Grant number: 13/09799-1
• Support Opportunities: Research Projects - Thematic Grants
• Start date: May 01, 2014
• End date: April 30, 2019
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: José Antunes Rodrigues
• Grantee: José Antunes Rodrigues
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Pesquisadores principais:( Últimos ): Ayrton Custodio Moreira ; Lucila Leico Kagohara Elias
• Pesquisadores principais:( Antigos ): Margaret de Castro
• Associated researchers: Margaret de Castro
• Associated research grant(s): 19/08621-0 - 2nd Pan American Congress of Physiological Sciences, AR.EXT
• Associated scholarship(s): 18/12311-4 - AgRP and POMC neurons and mesolimbic pathways in the control of feeding in neonatal nutritional programming. Ribeirão Preto 2018, BP.PD; 18/10920-3 - Effects of oxytocin (OT) and atrial natriuretic peptide (ANP) on retroperitoneal adipose tissue lipolysis in control and obese rats., BP.PD; 17/15156-7 - Participation of TCPTP in hypothalamic resistance to leptin induced by repeated exposure to endotoxin, BP.IC; + associated scholarships 16/20132-7 - Neuroendocrine control of hydromineral balance in obese rats, BP.IC; 14/07372-3 - Interaction of H2S, NO and CO gaseous systems on in vitro secretion of vasopressin, oxytocin and atrial natriuretic peptide by medial basal hypothalamus and neurohypophysis in response to extracellular hyperosmolality, BP.DR; 14/07240-0 - Involvement of endocannabinoids and glucocorticoids on the modulation of astrocytic function and in vitro humoral release in response to osmotic stimulation, BP.DR; 14/15218-4 - Energy balance and body fluid homeostasis control: from cells to the physiological systems, BP.PD; 14/10060-3 - Participation of intracellular pathways modulated by carbon monoxide in the regulation of hydroeletrolitic balance, BP.DR - associated scholarships

Abstract

The hypothalamus is essential for the maintainance of extracellular volume and tonicity, as well as energy homeostasis. In this study we aim to clarify the involvement of neuronal and glial components in the integrative responses, as well as the participation of key neurotransmitters, neuromodulators, hormones and neuropeptides in the neuroendocrine control of both water and sodium intake and excretion and food intake. Considering that maternal nutritional and hormonal factors strongly affect fetal/neonatal programming, which affects metabolic function in adult life, this study also aims to evaluate the effects of saline overload during pregnancy and lactation and its repercussions to the integrity of neuroendocrine responses in adulthood. We will also investigate the participation of central mediators of appetite and peripheral factors such as insulin, leptin, ghrelin, adiponectin, glucocorticoids and estrogen in energy homeostasis using experimental models of bilateral adrenalectomy, ovariectomy and endotoxin tolerance. The peak of glucocorticoid secretion occurs in accordance with feedind pattern, with interplay between hypothalamic-pituitary-adrenal axis activity, central nervous system and nutritional status. The hypothalamus acts as a integrative center of circadian rhythm and also of glucocorticoid secretion and energy homeostasis, integrating neuroendocrine and behavioral responses. Thus, in the present study, we will evaluate the expression of genes related with circadian rhythm and its correlation with orexigenic and anorexigenic neuropeptides. We will also evaluate the ontogeny of clock genes and diurnal secretion of corticosterone in rats. Therefore, this proposal, using in vivo and in vitro experimental models, aims to assess in an integrative view, from molecules to systems, the physiological and pathophysiological control of energy homeostasis and fluid balance. (AU)

Scientific publications

(26)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RORATO, RODRIGO; BORGES, BEATRIZ DE CARVALHO; UCHOA, ERNANE TORRES; ANTUNES-RODRIGUES, JOSE; ELIAS, CAROL FUZETI; KAGOHARA ELIAS, LUCILA LEICO. LPS-Induced Low-Grade Inflammation Increases Hypothalamic JNK Expression and Causes Central Insulin Resistance Irrespective of Body Weight Changes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 18, n. 7, JUL 2017. (13/09799-1, 12/18179-4)

VILHENA-FRANCO, TATIANE; VALENTIM-LIMA, EVANDRO; REIS, LUIS C.; ELIAS, LUCILA L. K.; ANTUNES-RODRIGUES, JOSE; MECAWI, ANDRE S.. Role of AMPA and NMDA receptors on vasopressin and oxytocin secretion induced by hypertonic extracellular volume expansion. Journal of Neuroendocrinology, v. 30, n. 8, SI, AUG 2018. (13/09799-1)

DOS-SANTOS, RAONI CONCEICAO; ISHIOKA, GABRIEL; COGNUCK, SUSANA QUIROS; MANTOVANI, MILENE; CALIMAN, IZABELA FACCO; KAGOHARA ELIAS, LUCILA LEICO; ANTUNES-RODRIGUES, JOSE. High-fat diet changes the behavioural and hormonal responses to water deprivation in male Wistar rats. Experimental Physiology, v. 107, n. 12, p. 13-pg., 2022-10-12. (13/09799-1, 16/20132-7, 18/18071-5, 19/01260-2)

COGNUCK, SUSANA QUIROS; REIS, WAGNER L.; SILVA, MARCIA S.; ALMEIDA-PEREIRA, GISLAINE; DEBARBA, LUCAS K.; ZORRO, V, SANDRA; MECAWI, ANDRE S.; FRANCI, CELSO R.; ELIAS, LUCILA L. K.; ANTUNES-RODRIGUES, JOSE. Sex- and age-dependent differences in the hormone and drinking responses to water deprivation. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, v. 318, n. 3, p. R567-R578, MAR 2020. (13/09799-1)

UCHOA, ERNANE TORRES; MARANGON, PAULA BEATRIZ; RORATO, RODRIGO; RUGINSK, SILVIA GRACIELA; DEBARBA, LUCAS KNIESS; ANTUNES-RODRIGUES, JOSE; ELIAS, LUCILA L. K.. Adrenalectomy impairs insulin-induced hypophagia and related hypothalamic changes. Journal of Endocrinology, v. 242, n. 2, p. 125-138, AUG 2019. (13/09799-1)

GREENWOOD, MICHAEL P.; GREENWOOD, MINGKWAN; ROMANOVA, ELENA V.; MECAWI, ANDRE S.; PATERSON, ALEX; SARENAC, OLIVERA; JAPUNDZIC-ZIGON, NINA; ANTUNES-RODRIGUES, JOSE; PATON, JULIAN F. R.; SWEEDLER, JONATHAN V.; et al. The effects of aging on biosynthetic processes in the rat hypothalamic osmoregulatory neuroendocrine system. NEUROBIOLOGY OF AGING, v. 65, p. 178-191, MAY 2018. (13/09799-1)

MOREIRA, AYRTON CUSTODIO; ANTONINI, SONIR RAUBER; DE CASTRO, MARGARET. A sense of time of the glucocorticoid circadian clock: from the ontogeny to the diagnosis of Cushing's syndrome. EUROPEAN JOURNAL OF ENDOCRINOLOGY, v. 179, n. 1, p. R1-R18, JUL 2018. (14/03989-6, 13/09799-1)

GONCALVES, GABRIEL HENRIQUE MARQUES; TRISTAO, SABRINA MARA; VOLPI, RAFAELLA EDUARDA; ALMEIDA-PEREIRA, GISLAINE; DE CARVALHO BORGES, BEATRIZ; DONATO, JR., JOSE; DE CASTRO, MARGARET; ANTUNES-RODRIGUES, JOSE; ELIAS, LUCILA LEICO KAGOHARA. STAT3 but Not ERK2 Is a Crucial Mediator Against Diet-Induced Obesity via VMH Neurons. Diabetes, v. 70, n. 7, p. 1498-1507, JUL 2021. (14/17248-8, 18/10090-0, 13/09799-1)

MONTEIRO, L. R. N.; MARANGON, P. B.; ELIAS, L. L. K.; REIS, L. C.; ANTUNES-RODRIGUES, J.; MECAWI, A. S.. Sodium appetite elicited by low-sodium diet is dependent on p44/42 mitogen-activated protein kinase (extracellular signal-regulated kinase 1/2) activation in the brain. Journal of Neuroendocrinology, v. 29, n. 9, SEP 2017. (13/09799-1)

BORGES, BEATRIZ C.; ELIAS, CAROL F.; ELIAS, LUCILA L. K.. PI3K signaling: A molecular pathway associated with acute hypophagic response during inflammatory challenges. Molecular and Cellular Endocrinology, v. 438, n. C, p. 36-41, DEC 15 2016. (13/03915-0, 13/09799-1)

DE ARAUJO, LEONARDO D.; ROA, SILVIA L.; BUENO, ANA C.; COELI-LACCHINI, FERNANDA B.; MARTINS, CLARISSA S.; UCHOA, ERNANE T.; ANTUNES-RODRIGUES, JOSE; ELIAS, LUCILA L.; ELIAS, PAULA C.; MOREIRA, AYRTON C.; et al. Restricted Feeding Schedules Modulate in a Different Manner the Expression of Clock Genes in Rat Hypothalamic Nuclei. FRONTIERS IN NEUROSCIENCE, v. 10, DEC 6 2016. (13/09799-1)

RIVAS, PRISCILA M. S.; VECHIATO, FERNANDA M. V.; BORGES, BEATRIZ C.; RORATO, RODRIGO; ANTUNES-RODRIGUES, JOSE; ELIAS, LUCILA L. K.. Increase in hypothalamic AMPK phosphorylation induced by prolonged exposure to LPS involves ghrelin and CB1R signaling. Hormones and Behavior, v. 93, p. 166-174, JUL 2017. (13/03915-0, 13/09799-1, 12/18179-4)

GREENWOOD, MINGKWAN; GREENWOOD, MICHAEL P.; MECAWI, ANDRE S.; LOH, SU YI; RODRIGUES, JOSE ANTUNES; PATON, JULIAN F. R.; MURPHY, DAVID. Transcription factor CREB3L1 mediates cAMP and glucocorticoid regulation of arginine vasopressin gene transcription in the rat hypothalamus. MOLECULAR BRAIN, v. 8, OCT 26 2015. (11/52108-4, 13/09799-1)

VILHENA-FRANCO, TATIANE; VALENTIM-LIMA, EVANDRO; REIS, LUIS C.; ELIAS, LUCILA L. K.; ANTUNES-RODRIGUES, JOSE; MECAWI, ANDRE S.. Role of AMPA and NMDA receptors on vasopressin and oxytocin secretion induced by hypertonic extracellular volume expansion. Journal of Neuroendocrinology, v. 30, n. 8, p. 7-pg., 2018-08-01. (13/09799-1)

VALENTIM-LIMA, EVANDRO; DE OLIVEIRA, JOSE ANTONIO CORTES; ANTUNES-RODRIGUES, JOSE.; REIS, LUIS CARLOS; GARCIA-CAIRASCO, NORBERTO; MECAWI, ANDRE S.. Neuroendocrine changes in the hypothalamic-neurohypophysial system in the Wistar audiogenic rat (WAR) strain submitted to audiogenic kindling. Journal of Neuroendocrinology, v. 33, n. 7, SI, JUL 2021. (19/05957-8, 14/50891-1, 14/15218-4, 13/09799-1)

DUTRA, V, SABRINA G.; PATERSON, ALEX; MONTEIRO, LIVIA R. N.; GREENWOOD, MICHAEL P.; GREENWOOD, MINGKWAN P.; AMARAL, LUDIMILA S.; MELO, MARIANA R.; COLOMBARI, DEBORA S. A.; COLOMBARI, EDUARDO; REIS, LUIS C.; et al. Physiological and Transcriptomic Changes in the Hypothalamic-Neurohypophysial System after 24 h of Furosemide-Induced Sodium Depletion. Neuroendocrinology, v. 111, n. 1-2, p. 70-86, DEC 2020. (14/15218-4, 13/23057-8, 13/09799-1, 19/08621-0)

PORCARI, CINTIA YAMILA; ARAUJO, IRACEMA GOMES; URZEDO-RODRIGUES, LILIA; DE LUCA, LAURIVAL ANTONIO; MENANI, JOSE VANDERLEI; CAEIRO, XIMENA ELIZABETH; IMBODEN, HANS; ANTUNES-RODRIGUES, JOSE; REIS, LUIS CARLOS; VIVAS, LAURA; et al. Whole body sodium depletion modifies AT1 mRNA expression and serotonin content in the dorsal raphe nucleus. Journal of Neuroendocrinology, v. 31, n. 4, APR 2019. (13/09799-1)

FLATOW, ELIZABETH A.; KOMEGAE, EVILIN N.; FONSECA, MONIQUE T.; BRITO, CAMILA F.; MUSTEATA, FLORIN M.; ANTUNES-RODRIGUES, JOSE; STEINER, ALEXANDRE A.. Elucidating the role of leptin in systemic inflammation: a study targeting physiological leptin levels in rats and their macrophages. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, v. 313, n. 5, p. R572-R582, NOV 2017. (16/04921-1, 13/09799-1, 14/03719-9, 12/03831-8)

QUIROS COGNUCK, SUSANA; REIS, WAGNER L.; SILVA, MARCIA; DEBARBA, LUCAS K.; MECAWI, ANDRE S.; DE PAULA, FRANCISCO J. A.; RODRIGUES FRANCI, CELSO; ELIAS, LUCILA L. K.; ANTUNES-RODRIGUES, JOSE. Sex differences in body composition, metabolism-related hormones, and energy homeostasis during aging in Wistar rats. PHYSIOLOGICAL REPORTS, v. 8, n. 20, OCT 2020. (13/09799-1)

RUIZ ROA, SILVIA LILIANA; MARTINEZ, EDSON ZANGIACOMI; MARTINS, CLARISSA SILVA; ANTONINI, SONIR RAUBER; DE CASTRO, MARGARET; MOREIRA, AYRTON CUSTODIO. Postnatal Ontogeny of the Circadian Expression of the Adrenal Clock Genes and Corticosterone Rhythm in Male Rats. Endocrinology, v. 158, n. 5, p. 1339-1346, MAY 2017. (12/22164-2, 13/09799-1)

UCHOA, ERNANE TORRES; RORATO, RODRIGO; RUGINSK, SILVIA GRACIELA; BORGES, BEATRIZ DE CARVALHO; ANTUNES-RODRIGUES, JOSE; ELIAS, LUCILA L. K.. Corticotrophin-releasing factor receptor 2 mediates the enhanced activation of satiety-related responses through oxytocin neurons in the paraventricular nucleus of the hypothalamus after adrenalectomy. Neuroscience Letters, v. 606, p. 123-128, OCT 8 2015. (13/09799-1)

GREENWOOD, MICHAEL P.; GREENWOOD, MINGKWAN; MECAWI, ANDRE S.; ANTUNES-RODRIGUES, JOSE; PATON, JULIAN F. R.; MURPHY, DAVID. Rasd1, a small G protein with a big role in the hypothalamic response to neuronal activation. MOLECULAR BRAIN, v. 9, JAN 7 2016. (11/52108-4, 13/09799-1)

VECHIATO, F. M. V.; RIVAS, P. M. S.; RUGINSK, S. G.; BORGES, B. C.; ELIAS, L. L. K.; ANTUNES-RODRIGUES, J.. The type-1 cannabinoid receptor modulates the hydroelectrolytic balance independently of the energy homeostasis during salt load. Hormones and Behavior, v. 78, p. 43-51, FEB 2016. (13/09799-1)

ALMEIDA-PEREIRA, G.; VILHENA-FRANCO, T.; COLETTI, R.; COGNUCK, S. Q.; SILVA, H. V. P.; ELIAS, L. L. K.; ANTUNES-RODRIGUES. 17 beta-Estradiol attenuates p38MAPK activity but not PKC alpha induced by angiotensin II in the brain. Journal of Endocrinology, v. 240, n. 2, p. 345-360, FEB 2019. (13/09799-1, 14/25005-8)

COGNUCK, S. QUIROS; REIS, W. L.; SILVA, M. S.; ZORRO, S., V; ALMEIDA-PEREIRA, G.; DEBARBA, L. K.; ELIAS, L. L. K.; ANTUNES-RODRIGUES, J.. Age and sex influence the response in lipid metabolism of dehydrated Wistar rats. SCIENTIFIC REPORTS, v. 12, n. 1, p. 13-pg., 2022-06-02. (13/09799-1)

RORATO, RODRIGO; BORGES, BEATRIZ DE CARVALHO; UCHOA, ERNANE TORRES; ANTUNES-RODRIGUES, JOSE; ELIAS, CAROL FUZETI; KAGOHARA ELIAS, LUCILA LEICO. LPS-Induced Low-Grade Inflammation Increases Hypothalamic JNK Expression and Causes Central Insulin Resistance Irrespective of Body Weight Changes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 18, n. 7, p. 14-pg., 2017-07-01. (13/09799-1, 12/18179-4)