Isolation and characterization of undifferentiated cells of embryonic eggs from Aedes aegypti (Linnaeus, 1762)

• Grant number: 13/07649-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2013
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Maria Angelica Miglino
• Grantee: Lara Carolina Mario
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The insects of the genus Aedes are animals warm or subtropical climate, being dispersed by the five continents the world, they are known to transmit dengue, an infectious disease transmitted by a virus single-stranded DNA, family Flaviviridae. The species Aedes aegypti, is characterized by a high rate adaptive near urban perimeters, which further enhances the case of the disease in these regions. Stem cells are undifferentiated cells or low degree of differentiation found in embryonic and extra embryonic tissues. Due to high demand of diseases caused by these insects, this research seeks to understand how their cellular organization, thus aiming at an alternative means of control thereof. To perform this work, stem cells from fertilized eggs will be collected, without infection of the smooth muscle of the digestive tract of Aedes insects, in order to isolate and characterize the stem cells. The cell line studied will come from breeding insects without infection, installed in UEMA, State University of Maranhão, Caxias, Maranhão. To accomplish this cultivation, the methods used are two, Grace and Sf900II. These, when appropriately supplemented with fetal bovine serum, may be employed in cultured insect cells. In order to deepen our knowledge in this area, the study of cell biology of Aedes insects, will give us a better view of how diseases that infect them are and will contribute to the biological control of these species. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARIO, LARA C.; BORGHESI, JESSICA; CRIVELLARI-DAMASCENO, WILSON T.; FAVARON, PHELIPE O.; CARREIRA, ANA CLAUDIA O.; WILL, SONIA E. A. L.; MARIA, DURVANEI A.; MIGLINO, MARIA A.. Egg and fourth instar larvae gut of Aedes aegypti as a source of stem cells. TISSUE & CELL, v. 48, n. 5, p. 558-565, OCT 2016. (13/07649-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARIO, Lara Carolina. Isolation and characterization of undifferentiated cells of embryonated eggs from Aedes aegypti (Linnaeus 1762). 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.