Development of Real Time RT-PCR for diagnosis of infections by Piry virus and other vesiculovirus

• Grant number: 13/02256-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2013
• End date: January 31, 2015
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Luiz Tadeu Moraes Figueiredo
• Grantee: Aline Lavado Tolardo
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The Vesículovirus (VSV) belonging to the family Rhabdoviridae. The genus VSV is subdivided into Indiana serotypes 1, Indiana 2 and Indiana 3, and subtype virus Piry is part of Indiana serotype 3. VSV infections and particularly those caused by Piry virus, produce acute febrile illness that is largely unknown by the human public health service in Brazil. Therefore, offering modern weapons diagnostic, consider that an investigation into the VSV and particularly about the Piry virus, it is necessary and should contribute to a better understanding of clinical and epidemiology of these viruses. We aim with this project detect virus genome of Piry and other VSV in serum of patients with acute febrile illness. To do so, we use conventionals RT-PCRs for VSV and a real-time RT-PCR will be developed as part of the Project and which will meet the titer of VSV in the clinical sample. Also, viruses whose genome is eventually detected will have their nucleotides sequenced for phylogenetic studies. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALINE LAVADO TOLARDO; WILLIAM MARCIEL DE SOUZA; MARILIA FARIGNOLI ROMEIRO; LUIZ CARLOS VIEIRA; LUCIANO KLEBER DE SOUZA LUNA; DYANA ALVES HENRIQUES; JANSEN DE ARAUJO; CARLOS EDUARDO HASSEGAWA SIQUEIRA; TATIANA ELIAS COLOMBO; VICTOR HUGO AQUINO; et al. A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus. Memórias do Instituto Oswaldo Cruz, v. 111, n. 6, p. 385-390, 2016-06-03. (13/02256-2, 13/14929-1, 12/02836-6, 12/24150-9)

ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; TOLARDO, ALINE LAVADO; VIEIRA, LUIZ CARLOS; HENRIQUES, DYANA ALVES; DE ARAUJO, JANSEN; HASSEGAWA SIQUEIRA, CARLOS EDUARDO; COLOMBO, TATIANA ELIAS; AQUINO, VICTOR HUGO; LOPES DA FONSECA, BENEDITO ANTONIO; et al. A real-time RT-PCR for rapid detection and quantification of mosquito-borne alphaviruses. ARCHIVES OF VIROLOGY, v. 161, n. 11, p. 3171-3177, NOV 2016. (13/02256-2, 12/02836-6, 13/14929-1, 12/24150-9, 14/20851-8)

ROMEIRO, MARILIA FARIGNOLI; DE SOUZA, WILLIAM MARCIEL; TOLARDO, ALINE LAVADO; VIEIRA, LUIZ CARLOS; COLOMBO, TATIANA ELIAS; AQUINO, VICTOR HUGO; NOGUEIRA, MAURICIO LACERDA; MORAES FIGUEIREDO, LUIZ TADEU. Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil. Revista da Sociedade Brasileira de Medicina Tropical, v. 49, n. 3, p. 279-285, MAY-JUN 2016. (13/02256-2, 12/02836-6, 14/20851-8, 12/24150-9, 13/14929-1)

DE SOUZA, WILLIAM MARCIEL; ACRANI, GUSTAVO OLSZANSKI; ROMEIRO, MARILIA FARIGNOLI; REIS JUNIOR, OSVALDO; TOLARDO, ALINE LAVADO; SERRAO DE ANDRADE, AMANDA ARAUJO; GONCALVES VIANEZ JUNIOR, JOAO LIDIO DA SILVA; DE ALMEIDA MEDEIROS, DANIELE BARBOSA; TEIXEIRA NUNES, MARCIO ROBERTO; MORAES FIGUEIREDO, LUIZ TADEU. Complete genome sequence of Piry vesiculovirus. ARCHIVES OF VIROLOGY, v. 161, n. 8, p. 2325-2328, AUG 2016. (12/24150-9, 13/14929-1, 14/20851-8, 13/02256-2, 14/02438-6, 15/05778-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TOLARDO, Aline Lavado. Development and application of a real-time RT-PCR for brazilian Vesiculovirus. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.