Study of asthma prevention with the administration of viable Saccharomyces cerevisiae in a murine model of allergic asthma

• Grant number: 13/02209-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2013
• End date: July 31, 2015
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Marcos de Carvalho Borges
• Grantee: Vanessa Maciel Braulio da Fonseca
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 10/20600-4 - Study of asthma prevention and treatment with the administration of viable and heat-killed Saccharomyces cerevisiae in a murine model of allergic asthma, AP.JP

Abstract

The prevalence of asthma has increased in children and in communities that adopt a Western lifestyle and become more urbanized. The hygiene hypothesis suggests that exposure to infections early in life can reduce the risk of asthma and other allergic diseases. Probiotics are live microorganisms that confer a health benefit to the host. Treatment with Saccharomyces cerevisiae protected mice against intestinal infections, prevented bacterial translocation and increased IL-10 production. Administration of Beta-1-3 Glucan, a polysaccharide extracted from fungi and yeasts such as S. cerevisiae, reduced symptoms of allergic rhinitis and increased IL-10 production in asthmatic children. There is no study of S. cerevisiae in the prevention or treatment of asthma. Objective: to evaluate the effects of viable S. cerevisiae oral administration in the prevention of asthma and the mechanisms involved. Methods: Balb/c mice will be used in the study. Viable S. cerevisiae will be administered before sensitization with ovalbumin. After sensitization and challenges, mice will be ventilated and in vivo measurements of bronchial hyperresponsiveness will be realized. Bronchoalveolar lavage will be collected to quantify total and differential cell counts and cytokine levels by ELISA (IL-4, IL-5, IL-10, IL-13, IFN-gama and TGF-beta). Histochemistry will be performed in the lungs to quantify inflammation, collagen and mucus. Real time PCR will be realized in lung tissue to measure inflammatory cytokine, such as IL-4, IL-5, IL-10, IL-13, IFN-gama and TGF-beta. Significance: Considering the hygiene hypothesis, the mechanisms of action and safety of probiotics, it is expected that administration of Saccharomyces cerevisiae will reduce airway inflammation, hyperresponsiveness and remodeling, and could be considered for asthma prevention. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FONSECA, Vanessa Maciel Braulio da. The administration of live Saccharomyces cerevisiae inhibits bronchial responsiveness and the airway inflammation in an animal model of asthma.. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.