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Assessment of the behavior of BTEX and its degradation metabolites in soils: studies in microcosms

Grant number: 12/13925-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2013
Effective date (End): June 30, 2015
Field of knowledge:Engineering - Sanitary Engineering
Principal researcher:Pedro Sergio Fadini
Grantee:Leidy Rocío Niño Camacho
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil


Contamination by polycyclic aromatic hydrocarbons such as benzene, toluene, ethylbenzene and xylenes, known as BTEX is commonly observed in areas near the oil refineries or stations, in water and soil. The anaerobic biodegradation in soil has been studied as a technology for recovering contaminated areas with anoxic characteristics, but, this microbial activity can transform compounds little or nothing toxic in highly toxic compounds, and in many cases, the products generated have cancerigenic characteristicsFaced with this situation, the present study has as main objective, the assessment of efficiency of the anaerobic biodegradation of BTEX in microcosms with respect to the production of metabolites that take part in this process, some of them: benzylsuccinate; etylbenzylsuccinate, 2-, 3-, and 4-metylbenzylsuccinate; toluate and benzoic acid. The BTEX will be extracted by HS-SPME and analyzed by GC /FID, the metabolites more hydrophilic and less volatile will be extracted by ultrasound and analyzed by UPLC. It is expected from monitoring the biodegradation kinetics, decrease of the concentration of the BTEX and the concentration increase of metabolites with time, contribute to an understanding of the dynamics of the process which can assist in making decisions and in the restoration of areas with incomplete degradation.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AWAN, ALMAS TAJ; NINO, LEIDY ROCIO; PAIX, MURILO D'ABRUZZO; MOZETO, ANTONIO APARECIDO; FADINI, PEDRO SERGIO. Urban Stream Vulnerability Toward PAHs and n-Alkanes and Their Source Identification. POLYCYCLIC AROMATIC COMPOUNDS, v. 38, n. 3, p. 294-309, 2018. Web of Science Citations: 1.
NINO, LEIDY R.; GROSSELI, GUILHERME M.; MOZETO, ANTONIO A.; FADINI, PEDRO S. Optimization study of BTEX extraction from soils spiked with two kinds of Brazilian diesel by HS SPME using Box-Behnken experimental design and multi-response analysis. ANALYTICAL METHODS, v. 6, n. 10, p. 3247-3253, 2014. Web of Science Citations: 7.

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