Study of cytokine secretion in a model of carrageenan-induced peritonitis in healthy and diabetic mice

• Grant number: 13/10307-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2013
• End date: July 31, 2014
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Joilson de Oliveira Martins
• Grantee: Maria Fernanda Casalechi Rezende
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 10/02272-0 - Effect of insulin on lung inflammation in animal with sepsis, innate immunit,activation of insulin gene (BGK) and insulin receptors (IR)-A and IR-B, AP.JP

Abstract

Diabetes mellitus is a metabolic disorder associated with an increased risk of infections affecting the mucous membranes and soft tissues. Experimental studies have demonstrated that diabetic animals have reduced adhesion and migration of cells to the inflammatory site. This decrease is following by low levels of secretion of TNF-±, IL-1², IL-6, and IL-10 as well as low expression of ICAM-1. It should be emphasized that the treatment of diabetic animals with insulin reverses these effects observed on cell adhesion and migration, corroborating the literature reporting the immunomodulatory role of insulin. Thus, this project aims to assess the role of insulin on the secretion of cytokines, important chemical mediators involved in the inflammatory response induced by carrageenan in animals healthy and diabetic (alloxan, 60 mg/kg, iv), treated or not with insulin. For that it will be evaluated: a) blood glucose (glucose monitor), b) the number of peritoneal cells lavage (PeL), b) concentrations of cytokines (IL-1², TNF-±, IL-6, IFN-³, IL-4, IL-10, CINC-1, and CINC-2) in PeL (ELISA). (AU)