Effect of traumatic occlusion in the number of osteoclast and bone density of the alveolar bone

• Grant number: 12/22432-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2013
• End date: December 31, 2013
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Daniela Atili Brandini
• Grantee: Marina Fuzette Amaral
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The most recent paper that explores the relationship between traumatic occlusion and periodontal disease, points out the necessity of some more studies to explain this association. In the face of occlusal trauma, morphofunctional alterations are observed, such as disorientation and a decrease in collagenous fibers, change in the alignment of periodontal fibers, an increase in the number of fibroblasts, elevated osteoclast activity, venous thrombosis, and cell necrosis in the periodontal ligament. Objective: This study aims to evaluate the effect of occlusal trauma in the alveolar bone in rat molars through quantitative analyses of the number of osteoclast around the roots and in the alveolar bone between the roots, and bone density in the alveolar bone between the roots of the first upper molar. Material and Method: For this study, 40 male Wistar rats of seven weeks old will be divided into the Control group and the Traumatic occlusion group. The periods of study will be 2, 5, 7, and 14 days. In the experimental group, the inferior right first molar will be raised by direct filling; creating a flat high occlusal table at the highest occlusal cusp. Transversal histological sections of 5 mm wide of the right upper first molar and surrounding tissues will be stained with hematoxylin and eosin, and histochemical treatment for TRAcP detection. The sections counterstained with HE will be used for analyzes of the alveolar bone density, and the sections that will be submitted to histochemical treatment for TRAcP detection will be used to count the number of osteoclasts. The data will be analyzed using IBM SPSS 20.0 at =0.05. The one-way analysis of variance with Bonferroni post-hoc-test or Kruskal-Wallis test will be used for group comparison. Data will be expressed as mean ± SD or percentage. (AU)