Study of antiviral action of Phyllocaulis boraceiensis mucus

• Grant number: 12/22906-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2013
• End date: July 31, 2016
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Ronaldo Zucatelli Mendonca
• Grantee: Ana Rita de Toledo Piza
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Terrestrial gastropods exude mucus by the body surface, when traveling, to protect its body from mechanical injury, desiccation or contact with harmful substances. Mucus of molluscs has been studied as a source of new natural compounds with diverse biological activities, as ability to induce cell proliferation. Human fibroblasts treated with 0.012 mg/µl of Phyllocaulis boraceiensis mucus have high rates of proliferation inducing a significant increase in production and secretion of extracellular matrix such as collagen fibres. Studies involving the mucus of P. boraceiensis can elucidate the presence of proteins that induces healing process in mice submitted a dorsal incision. The aim of this project is to identify, isolate, purify and sequence proteins present in the mucus of the land slug P. boraceiensis with antiviral action "in vitro". Applying proteomic techniques as two-dimensional gels, fragmentation by chromatography and mass spectrometry. The viral activity will be determined "in vitro" using VERO, L929 and MDCK cells infected with measles virus, poliovirus and Influenza virus.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE TOLEDO-PIZA, ANA RITA; DE OLIVEIRA, MARIA ISABEL; NEGRI, GIUSEPPINA; MENDONCA, RONALDO ZUCATELLI; FIGUEIREDO, CRISTINA ADELAIDE. Polyunsaturated fatty acids from Phyllocaulis boraceiensis mucus block the replication of influenza virus. Archives of Microbiology, v. 200, n. 6, p. 961-970, AUG 2018. (12/22906-9, 12/22555-1)

DE TOLEDO-PIZA, ANA RITA; DE OLIVEIRA, MARIA ISABEL; NEGRI, GIUSEPPINA; MENDONCA, RONALDO ZUCATELLI; FIGUEIREDO, CRISTINA ADELAIDE. Polyunsaturated fatty acids from Phyllocaulis boraceiensis mucus block the replication of influenza virus. Archives of Microbiology, v. 200, n. 6, p. 10-pg., 2018-08-01. (12/22906-9, 12/22555-1)