| Grant number: | 13/02974-2 |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| Start date: | September 01, 2013 |
| End date: | August 31, 2014 |
| Field of knowledge: | Health Sciences - Physiotherapy and Occupational Therapy |
| Principal Investigator: | Rinaldo Roberto de Jesus Guirro |
| Grantee: | Marcos Ferracioli Santos |
| Host Institution: | Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
Abstract Infections caused by Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli are among the most frequent being those in hospitals, the bacteria that most commonly colonize pressure ulcers. The use of physical agents such as low level laser therapy (LLLT) has been described as adjuvant therapeutic methods to inhibit the growth of microorganisms, however there is no consensus in the best treatment to be used. The purpose of this study is to analyze the influence of the laser on the rate and growth curve of bacteria in vitro, the strains of S. aureus ATCC 25923 (Gram +), P. aeruginosa ATCC 27853 (Gram-) and E. coli ATCC 25922 (Gram-). Upon activation of the strains, the cells are suspended in saline and the concentration adjusted to 103 cells ml-1. The crops will be sown in liquid medium and irradiated with the laser at a wavelength of 450 and 904nm, the fluences of 0 (control), 3, 6, 12, 18, and 24J/cm2 and incubated at 37°C. The colony-forming units (CFU / mL) will be counted after the irradiation times of 24, 48 and 72 hours. The results will be submitted to a linear regression model with mixed effects (random and fixed effects), with p<0,05 | |
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