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Immunohistochemical profile of canine Diffuse Large B Cell Lymphoma using the method of tissue microarray (TMA)

Grant number: 13/02253-3
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2013
Effective date (End): May 31, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Julio Lopes Sequeira
Grantee:Maria Claudia Lopes da Silva
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Non Hodgkin lymphomas (LNHs) are the most common hematopoietic tumor of dogs, among which Diffuse Large B Cell Lymphoma (DLBCL) is the most frequent subtype in the dog and man. DLBCLs are tumors composed of lymphoid B cell characterized by a diffuse growth pattern. DLBCLs may present at least five variants which relates to different patterns of biological behavior including in dogs. However, routinely these tumors are only classified as DLBCLs without taking into account its morphologic characteristics, immunohistochemical profile and proliferation index. Because of that the World Health Organization (WHO) proposes that on research projects those variants are thoroughly investigated in order to identify differences that enable establishment of new entities. The present work objects to delineate the immunohistochemical profile of Diffuse Large B Cell Lymphoma of dogs using the method of TMA (tissue microarray) and analyze them according to the following proposals of those tumor classifications: World Health Organization (WHO, 2008); Classification of Hematopoietic Tumors of Domestic Animals (WHO, 2002) and Updated Kiel Classification (LENNERT e FELLER, 1992). The evaluations will include the verification of the immunohistochemical expression of different types of pan B markers (anti CD79a, anti CD20 and PAX-5); the determination of proliferation index (immunohistochemical expression of Ki-67) and apoptosis (immunohistochemical expression of caspase 3) and also the expression of p53. All evaluations will be performed using conventional histological sections and those obtained through tissue microarray technique.